基于GC-MS和GC-IMS联用法分析不同采收期广佛手精油挥发性成分

doi:10.7506/spkx1002-6630-20200618-244

食品科学 ›› 2021, Vol. 42 ›› Issue (16): 193-202.doi: 10.7506/spkx1002-6630-20200618-244

基于GC-MS和GC-IMS联用法分析不同采收期广佛手精油挥发性成分

汤酿，刘静宜，陈小爱，杨玉洁，陈树喜，张子明，周爱梅

（1.华南农业大学食品学院，广东省功能食品活性物重点实验室，广东广州 510642；2.广东展翠食品股份有限公司，广东潮州 515634）

发布日期:2021-08-27
基金资助:
广东省“扬帆计划”引进创新创业团队项目（2016YT03S056）

Analysis of Volatile Components in Essential Oil of Fingered Citrons Citrus medica L. var. sarcodactylis Swingle Harvested in Guangdong Province at Different Times by GC-MS and GC-IMS

TANG Niang, LIU Jingyi, CHEN Xiao’ai, YANG Yujie, CHEN Shuxi, ZHANG Ziming, ZHOU Aimei

(1. Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou 510642, China; 2. Guangdong Zhancui Food Co. Ltd., Chaozhou 515634, China)

Published:2021-08-27

摘要/Abstract

摘要： 以不同采收期广佛手提取的精油为研究对象，采用气相色谱-质谱（gas chromatography-mass spectrometry，GC-MS）和气相色谱离子迁移谱（gas chromatography-ion mobility spectrometry，GC-IMS）技术分析广佛手精油含量和挥发性成分的变化。结果表明，广佛手精油含量在9月达到峰值。GC-MS和GC-IMS技术在5?个采收期广佛手精油中分别鉴定出54?种和61?种挥发性成分，主要为萜烯类，其次为醇类、醛类和酯类等化合物。GC-MS结果表明，醇类、醛类、酯类、烷烃类和酚类物质主要存在于8月、9月样品中，且含量较少。GC-IMS结果表明，醛类、酯类和酸类化合物主要存在于6月、7月和8月样品中。通过热图聚类和主成分分析化学计量法对5?个采收期广佛手精油进行区分。2?种技术的检测结果呈现一定的差异性，GC-MS技术检测出的大部分为大分子（C9～C21）高含量挥发性成分，而GC-IMS检测出的多为小分子（C2～C10）低含量挥发性成分。2?种技术相结合扩大了样品中挥发性组分的检测范围，更加全面直观地反映广佛手精油中挥发性成分的变化情况，为广佛手精油品质鉴别和其最佳采收时间的选取提供科学依据。

关键词: 气相色谱-质谱联用；气相色谱-离子迁移谱；挥发性成分；不同采收期；广佛手精油

Abstract: In this study, the essential oil content and composition of fingered citrons harvested in Guangdong province in different months of the same year were analyzed by gas chromatography-mass spectrometry (GC-MS) and gas chromatography-ion mobility spectrometry (GC-IMS). The results showed that the essential oil content of fingered citrons peaked in September. GC-MS and GC-IMS identified 54 and 61 volatile components in the essential oil of finger citrons harvested at five different times, respectively, with terpenes being the dominant ones, followed by alcohols, aldehydes and esters. GC-MS indicated that alcohols, aldehydes, esters, alkanes and phenols mainly existed in the samples harvested in August and September at low levels. GC-IMS demonstrated that aldehydes, esters and acid compounds were mainly found in the samples harvested in June, July and August. Two chemometric methods, heat map clustering and principal component analysis, were used to distinguish the essential oils from fingered citrons harvested at five different times. The results of GC-MS and GC-IMS showed differences from each other. Most of the volatile components detected by GC-MS were larger molecules (C9–C21) at high levels, while most of the volatile components detected by GC-IMS were smaller molecules (C2–C10) at low levels. The combined application of both technologies expanded the scope of detectable volatile components in the essential oil from fingered citrons and could provide a scientific basis for the quality identification of the essential oil and the selection of optimal harvest time for fingered citrons.

Key words: gas chromatography-mass spectrometry; gas chromatography-ion mobility spectrometry; volatile compounds; different harvest times; essential oil of fingered citrons from Guangdong province

中图分类号:

TS255.1

汤酿，刘静宜，陈小爱，杨玉洁，陈树喜，张子明，周爱梅. 基于GC-MS和GC-IMS联用法分析不同采收期广佛手精油挥发性成分[J]. 食品科学, 2021, 42(16): 193-202.

TANG Niang, LIU Jingyi, CHEN Xiao’ai, YANG Yujie, CHEN Shuxi, ZHANG Ziming, ZHOU Aimei. Analysis of Volatile Components in Essential Oil of Fingered Citrons Citrus medica L. var. sarcodactylis Swingle Harvested in Guangdong Province at Different Times by GC-MS and GC-IMS[J]. FOOD SCIENCE, 2021, 42(16): 193-202.

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基于GC-MS和GC-IMS联用法分析不同采收期广佛手精油挥发性成分

Analysis of Volatile Components in Essential Oil of Fingered Citrons Citrus medica L. var. sarcodactylis Swingle Harvested in Guangdong Province at Different Times by GC-MS and GC-IMS

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