人工模拟胃肠道模型在食源性致病菌异质性研究中的应用进展

doi:10.7506/spkx1002-6630-20201024-240

食品科学 ›› 2021, Vol. 42 ›› Issue (23): 268-274.doi: 10.7506/spkx1002-6630-20201024-240

人工模拟胃肠道模型在食源性致病菌异质性研究中的应用进展

赵伟，王扬眉，潘迎捷，赵勇，刘海泉

（1.上海海洋大学食品学院，上海 201306；2.上海水产品加工及贮藏工程技术研究中心，上海 201306；3.农业农村部水产品贮藏保鲜质量安全风险评估实验室（上海），上海 201306；4.上海海洋大学食品热加工工程技术研究中心，上海 201306）

出版日期:2021-12-15 发布日期:2021-12-29
基金资助:
国家自然科学基金面上项目（31671779）；“十三五”国家重点研发计划重点专项（2018YFC1602205；2018YFC160220）；上海市教育委员会科研创新计划项目（2017-01-07-00-10-E00056）

Progress in the Application of Simulated Gastrointestinal Models to Study the Heterogeneity of Foodborne Pathogens

ZHAO Wei, WANG Yangmei, PAN Yingjie,, ZHAO Yong, LIU Haiquan

(1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China;2. Shanghai Aquatic Products Processing and Storage Engineering Technology Research Center, Shanghai 201306, China;3. Laboratory of Quality & Safety Risk Assessment for Aquatic Product on Storage and Preservation (Shanghai), Ministry of Agriculture and Rural Affairs, Shanghai 201306, China;4. Engineering Research Center of Food Thermal-Processing Technology, Shanghai Ocean University, Shanghai 201306, China)

Online:2021-12-15 Published:2021-12-29

摘要/Abstract

摘要： 探究食源性致病菌在人体胃肠道环境中的异质性以及与肠道菌群间的相互作用对食源性致病菌的控制和预防具有十分重要的意义。人工模拟胃肠道模型基于人体胃肠道的生理过程，在体外条件下模拟体内的消化吸收情况，可以部分或完全替代活体实验，是研究食源性致病菌异质性以及致病机理的一种重要工具。本文系统综述应用人工模拟胃肠道模型研究食源性致病菌在胃肠道中的耐受、耐药异质性，为研究食源性致病菌在人体胃肠道中的异质性提供理论参考，同时将其与食源性致病菌动物感染模型（体内胃肠道模型）进行比较，综合评价人工模拟胃肠道模型在食源性致病菌异质性研究中的应用，以期为构建更加全面、科学的食品安全风险评估体系提供一定指导。

关键词: 食源性致病菌；异质性；感染模型；人工模拟胃肠道模型

Abstract: Exploring the heterogeneity and growth of foodborne pathogenic bacteria in the human gastrointestinal tract and their interaction with the intestinal flora is of great significance for the control and prevention of foodborne pathogens. Artificial gastrointestinal tract models are designed based on the physiological processes taking place in the human gastrointestinal tract to simulate digestion and absorption in the body, which can partially or completely replace in vivo experiments. Therefore, the models are an important tool for studying the heterogeneity and pathogenic mechanism of foodborne pathogenic bacteria. The present article systematically reviews the application of simulated gastrointestinal models to study the heterogeneity of tolerance and drug resistance in foodborne pathogens in the gastrointestinal tract, which may provide a theoretical basis for studying the heterogeneity of foodborne pathogens in the human gastrointestinal tract. Besides, simulated gastrointestinal tract models are compared with animal models of infection with foodborne pathogens (in vivo gastrointestinal tract models). Finally, we comprehensively evaluate the application of simulated gastrointestinal tract models in the study of the heterogeneity of foodborne pathogens. We hope that this review will guide researches in building a more comprehensive and scientific food safety risk assessment system.

Key words: foodborne pathogens; heterogeneity; infection model; simulated gastrointestinal tract model

中图分类号:

TS201.3

赵伟，王扬眉，潘迎捷，赵勇，刘海泉. 人工模拟胃肠道模型在食源性致病菌异质性研究中的应用进展[J]. 食品科学, 2021, 42(23): 268-274.

ZHAO Wei, WANG Yangmei, PAN Yingjie,, ZHAO Yong, LIU Haiquan. Progress in the Application of Simulated Gastrointestinal Models to Study the Heterogeneity of Foodborne Pathogens[J]. FOOD SCIENCE, 2021, 42(23): 268-274.

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人工模拟胃肠道模型在食源性致病菌异质性研究中的应用进展

Progress in the Application of Simulated Gastrointestinal Models to Study the Heterogeneity of Foodborne Pathogens

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