具有保护肠上皮细胞HT-29免受大肠杆菌和H2O2损伤潜力的植物乳杆菌的筛选

doi:10.7506/spkx1002-6630-20210421-299

食品科学 ›› 2022, Vol. 43 ›› Issue (5): 143-150.doi: 10.7506/spkx1002-6630-20210421-299

具有保护肠上皮细胞HT-29免受大肠杆菌和H2O2损伤潜力的植物乳杆菌的筛选

马长路，柳青，李淑荣，张丽娟，妥彦峰

（1.北京农业职业学院食品与生物工程系，北京 102442；2.大连工业大学食品学院，辽宁大连 116034）

发布日期:2022-03-28
基金资助:
北京市农业科技项目（20200124）；北京农业职业学院科技援助项目（XY-BZ-20-01）

Screening Lactobacillus plantarum Strains with Potential to Protect Human Intestinal Epithelium HT-29 Cells against Injury Caused by Escherichia coli or H2O2

MA Changlu, LIU Qing, LI Shurong, ZHANG Lijuan, TUO Yanfeng

(1. Department of Food and Biological Engineering, Beijing Vocational College of Agriculture, Beijing 102442, China; 2. School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China)

Published:2022-03-28

摘要/Abstract

摘要： 益生菌可在肠道定植从而发挥抗炎或抗氧化活性，有利于宿主肠道健康。本实验研究了从新疆传统发酵乳制品中分离得到的8?株植物乳杆菌对大肠杆菌侵袭和过氧化氢刺激肠上皮细胞HT-29的保护作用。结果表明：在8?株植物乳杆菌中，植物乳杆菌35具有最高的黏附能力。植物乳杆菌35可通过取代、竞争、排阻的方式抑制大肠杆菌对HT-29细胞的黏附，抑制率分别为42.60%、59.17%、60.19%。植物乳杆菌35及其多糖可抑制大肠杆菌刺激HT-29细胞产生白细胞介素-8；同时保护HT-29细胞免受过氧化氢的损伤，增加超氧化物歧化酶、谷胱甘肽过氧化物酶活力水平并降低丙二醛含量。结论：植物乳杆菌35及其粗胞外多糖具有抑制大肠杆菌O157诱导的炎症性肠病的潜力。

关键词: 乳杆菌；抑制黏附；大肠杆菌O157；人结肠癌细胞HT-29

Abstract: Probiotic Lactobacillus strains can exert anti-inflammatory or antioxidant activities after colonization in the gut, which is beneficial for the host’s intestinal health. In this study, eight L. plantarum strains isolated from traditional fermented dairy foods in Xinjiang, China, were evaluated for their protective effects on HT-29 human intestinal epithelial cells against injury caused by Escherichia coli or hydrogen peroxide (H2O2). Among these strains, L. plantarum 35 showed the highest adhesion capacity to HT-29 cell monolayers, and it reduced E. coli adhesion to HT-29 cells by displacement, competition, and exclusion, with inhibition rates of 42.60%, 59.17%, and 60.19%, respectively. L. plantarum 35 and its crude exopolysaccharide (EPS) inhibited E. coli from stimulating HT-29 cells to produce interleukin-8 (IL-8), protected HT-29 cells against injury induced by H2O2, increased the levels of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and decreased malondialdehyde (MDA) content in HT-29 cells. Therefore, L. plantarum 35 and its crude EPS have the potential to inhibit inflammatory bowel disease induced by E. coli O157.

Key words: Lactobacillus; anti-adhesion; Escherichia coli O157; HT-29 cells

中图分类号:

TS201.6

马长路，柳青，李淑荣，张丽娟，妥彦峰. 具有保护肠上皮细胞HT-29免受大肠杆菌和H2O2损伤潜力的植物乳杆菌的筛选[J]. 食品科学, 2022, 43(5): 143-150.

MA Changlu, LIU Qing, LI Shurong, ZHANG Lijuan, TUO Yanfeng. Screening Lactobacillus plantarum Strains with Potential to Protect Human Intestinal Epithelium HT-29 Cells against Injury Caused by Escherichia coli or H2O2[J]. FOOD SCIENCE, 2022, 43(5): 143-150.

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具有保护肠上皮细胞HT-29免受大肠杆菌和H2O2损伤潜力的植物乳杆菌的筛选

Screening Lactobacillus plantarum Strains with Potential to Protect Human Intestinal Epithelium HT-29 Cells against Injury Caused by Escherichia coli or H2O2

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