食品科学 ›› 2022, Vol. 43 ›› Issue (6): 212-220.doi: 10.7506/spkx1002-6630-20210712-121

• 生物工程 • 上一篇    下一篇

固态发酵对麸皮抗氧化特性及全麦面包感官品质的提升作用

裴斐,杜逸飞,孙磊,李文,方勇,胡秋辉   

  1. (南京财经大学食品科学与工程学院,江苏省现代粮食流通与安全协同创新中心,江苏高校粮油质量安全控制及深加工重点实验室,江苏南京 210023)
  • 出版日期:2022-03-25 发布日期:2022-03-28
  • 基金资助:
    江苏高校优势学科建设工程资助项目(PAPD)

Effect of Solid-state Fermentation on Antioxidant Properties of Wheat Bran and Sensory Quality of Whole Wheat Bread

PEI Fei, DU Yifei, SUN Lei, LI Wen, FANG Yong, HU Qiuhui   

  1. (Key Laboratory of Grains and Oils Quality Control and Processing, Collaborative Innovation Center for Modern Grain Circulation and Safety, College of Food Science and Engineering, Nanjing University of Finance, Nanjing 210023, China)
  • Online:2022-03-25 Published:2022-03-28

摘要: 用植物乳杆菌LB-1和酿酒酵母对小麦麸皮进行固态发酵,利用发酵麸皮重组全麦粉制备全麦面包,并对固态发酵麸皮的抗氧化特性及全麦面包的色泽、组织结构、风味、口感等感官品质进行分析。结果表明:经过乳酸菌LB-1与酵母菌协同发酵36?h后,麸皮的总游离氨基酸、可溶性蛋白、可溶性多酚和可溶性阿拉伯木聚糖分别增加了0.44、0.82、1.67、1.68?倍。发酵麸皮的总还原力、DPPH自由基清除率以及超氧阴离子自由基清除率分别提升了0.88、0.42?倍和2.1?倍。将发酵麸皮制成全麦面包后,与未发酵组相比,协同发酵麸皮后全麦面包芯和面包皮L*值显著降低,其中,协同发酵麸皮36?h后制得全麦面包比容增加了0.24?倍,并且硬度、胶着度和咀嚼度显著降低,内聚性和弹性显著增加,表明乳酸菌LB-1与酵母菌协同发酵36?h组全麦面包具备更加松软、适口的口感。此外,固态发酵使全麦面包增加了15?种挥发性风味化合物,其中乙酸和乙醇的含量显著提升,全麦面包的风味特性得到增强。其中,36?h发酵组的全麦面包感官评分最高。因此,小麦麸皮在经过乳酸菌LB-1与酵母菌协同固态发酵36?h后,能够有效提升其体外抗氧化活性,降低全麦面包中淀粉的老化速度,从而改善全麦面包黏弹性等质构特性。并且改善全麦面包的色泽、组织结构、风味、口感等感官品质,具有良好的应用潜力。

关键词: 乳酸菌;固态发酵;麸皮;抗氧化特性;全麦面包;感官品质

Abstract: Solid-state fermented wheat bran with Lactobacillus plantarum LB-1 and Saccharomyces cerevisiae was used to prepare whole wheat bread. The antioxidant properties of fermented bran and the sensory quality of whole wheat bread such as color, specific volume, texture, flavor and mouthfeel were analyzed. The results showed that the contents of total free amino acids, soluble protein, soluble polyphenols and soluble arabinoxylan in wheat bran increased by 1.44, 1.82, 2.67 and 2.68 times after 36 h mixed culture fermentation, respectively. The total reducing power, 1,1-dipheny1-2-picryl-hydrazyl (DPPH) radical and superoxide anion radical scavenging capacity increased by 1.88, 1.42 and 3.1 times, respectively. The L* values of whole wheat bread crumb and crust with fermented bran decreased significantly as compared to those with unfermented bran. Moreover, addition of bran fermented for 36 h increased bran bread specific volume by 1.24 times, decreased significantly hardness, gumminess and chewiness, and cohesiveness and elasticity increased significantly, resulting in a more soft and palatable mouthfeel. In addition, solid-state fermentation of wheat bran produced 15 new volatile flavor compounds, and increased the contents of acetic acid and ethanol significantly, thus enhancing the flavor characteristics of whole wheat bread. The sensory score of whole wheat bread with bran fermented for 36 h was the highest. In conclusion, 36 h solid-state fermentation by Lactobacillus LB-1 and yeast effectively enhances the antioxidant activity in vitro of wheat bran, slows down the aging of starch in whole wheat bread and consequently improves texture properties including viscoelasticity as well as sensory attributes such as color, flavor and taste, and thus it has great application potential.

Key words: lactic acid bacteria; solid-state fermentation; bran; antioxidant properties; whole wheat bread; sensory quality

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