食品科学 ›› 2022, Vol. 43 ›› Issue (6): 337-346.doi: 10.7506/spkx1002-6630-20210121-233

• 安全检测 • 上一篇    下一篇

脂质氧化加速实验3 种方法的比较分析

杨壹芳,罗曼妮,胡秋红,朱勋存,李琦,张林,余沁芯,肖子涵,杨勇   

  1. (四川农业大学食品学院,四川雅安 625000)
  • 出版日期:2022-03-25 发布日期:2022-03-28
  • 基金资助:
    四川省科技厅项目(2020YJ0347);四川农业大学学科建设基金项目(03572094)

Comparative Analysis of Three Accelerated Lipid Oxidation Methods

YANG Yifang, LUO Manni, HU Qiuhong, ZHU Xuncun, LI Qi, ZHANG Lin, YU Qinxin, XIAO Zihan, YANG Yong   

  1. (College of Food Science, Sichuan Agricultural University, Ya’an 625000, China)
  • Online:2022-03-25 Published:2022-03-28

摘要: 以猪背膘为实验对象,研究比较高温加热、反复冻融及紫外照射3 种加速脂质氧化方法的效果。通过分析酸价、游离脂肪酸含量及组成、过氧化值(peroxide value,POV)、硫代巴比妥酸反应物(thiobarbituric acid reactive substances,TBARS)值及脂质氧化产物等指标的变化情况,同时结合测定微生物的数量,获得最适宜的脂质氧化加速方法。结果表明:高温加热处理的氧化效果最佳,猪背膘的POV从(0.008?3±0.00)g/100?g升至(0.053?0±0.00)g/100?g,TBARS值从(0.24±0.01)mg/kg升至(0.65±0.00)mg/kg;反复冻融处理的氧化效果次之,猪背膘的POV从(0.018?0±0.00)g/100?g升至(0.034?7±0.00)g/100?g,TBARS值从(0.13±0.00)mg/kg升至(0.25±0.00)mg/kg;紫外照射处理的氧化效果最差,猪背膘的POV从(0.008?1±0.00)g/100?g升至(0.019?8±0.00)g/100?g,TBARS值从(0.07±0.00)mg/kg升至(0.21±0.00)mg/kg。在高温加热处理的高温组中没有微生物检出;在反复冻融及紫外照射处理中,猪背膘中微生物数量均随着冻融次数增加或照射时间延长而逐渐增加,这不能排除微生物生长对猪肥膘脂质氧化的影响。因此,在这3?种方法中,高温加热处理是加速脂质氧化最适宜的方法。

关键词: 高温加热处理;反复冻融处理;紫外照射处理;脂质氧化;微生物数量

Abstract: The effects of high-temperature heating, repeated freezing and thawing, and ultraviolet (UV) irradiation on the acceleration of lipid oxidation in pork backfat were compared. Changes in the acid value (AV), free fatty acid (FFA) content and composition, peroxide value (POV), thiobarbituric acid reactive substances (TBARS) value, lipid oxidation products (LOP) and microbial load of pig backfat were measured to obtain the most suitable accelerated lipid oxidation method. The results showed that high-temperature heating treatment was the most effective way to promote lipid oxidation, increasing the POV from (0.008 3 ± 0.00) to (0.053 0 ± 0.00) g/100 g and the TBARS value from (0.24 ± 0.01) to (0.65 ± 0.00) mg/kg. Repeated freezing-thawing treatment increased the POV from (0.018 0 ± 0.00) to (0.034 7 ± 0.00) g/100 g and the TBARS value from (0.13 ± 0.00) to (0.25 ± 0.00) mg/kg, and UV irradiation increased the POV from (0.008 1 ± 0.00) to (0.019 8 ± 0.00) g/100 g and the TBARS value from (0.07 ± 0.00) to (0.21 ± 0.00) mg/kg. No microorganisms were detected in the high-temperature heating treatment. In the repeated freezing-thawing and UV irradiation treatments, the number of microorganisms gradually increased with the number of freezing-thawing cycles or irradiation time. This does not rule out the influence of microbial growth on lipid oxidation. Therefore, high-temperature heating treatment is the most suitable method to accelerate lipid oxidation among these three methods.

Key words: high-temperature heating treatment; repeated freezing-thawing treatment; ultraviolet irradiation treatment; lipid oxidation; number of microorganisms

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