动物双歧杆菌乳亚种XLTG11对葡聚糖硫酸钠诱导小鼠溃疡性结肠炎的缓解作用

doi:10.7506/spkx1002-6630-20210829-373

食品科学 ›› 2022, Vol. 43 ›› Issue (17): 164-173.doi: 10.7506/spkx1002-6630-20210829-373

动物双歧杆菌乳亚种XLTG11对葡聚糖硫酸钠诱导小鼠溃疡性结肠炎的缓解作用

马岩，王中江，杨靖瑜，李哲，彭霞，陈昱凤，李柏良

（1.沈阳师范大学实验教学中心，辽宁沈阳 110034；2.东北农业大学食品学院，黑龙江哈尔滨 150030）

出版日期:2022-09-15 发布日期:2022-09-28
基金资助:
辽宁省科技厅重点研发计划项目（2020JH2/10200039）；沈阳市科技特派团项目（20-207-3-46）

Alleviating Effect of Bifidobacterium animalis subsp. lactis XLTG11 on Ulcerative Colitis Induced by Sodium Dextran Sulfate in Mice

MA Yan, WANG Zhongjiang, YANG Jingyu, LI Zhe, PENG Xia, CHEN Yufeng, LI Bailiang

(1. Experimental Teaching Center, Shenyang Normal University, Shenyang 110034, China;2. College of Food Science, Northeast Agricultural University, Harbin 150030, China)

Online:2022-09-15 Published:2022-09-28

摘要/Abstract

摘要： 目的：通过葡聚糖硫酸钠（dextran sulfate sodium，DSS）诱导小鼠溃疡性结肠炎模型，探究动物双歧杆菌乳亚种XLTG11对小鼠溃疡性结肠炎的缓解作用。方法：将45 只8 周龄C57BL/6N雄鼠随机分为3 组：正常组、模型组和双歧杆菌组，小鼠自由摄入3%（质量分数）DSS水溶液，建立溃疡性结肠炎模型，双歧杆菌组灌胃动物双歧杆菌乳亚种XLTG11（1×107 CFU/d），测定小鼠体质量变化率、结肠长度、疾病活动指数（disease activity index，DAI）和肠髓过氧化物酶（myeloperoxidase，MPO）活力，观察结肠组织病理学变化，测定结肠肿瘤坏死因子（tumor necrosis factor，TNF）-α、白细胞介素（interleukin，IL）-6、IL-1β、IL-10质量浓度，测定肠道菌群组成及短链脂肪酸含量，分析肠道屏障和核转录因子（nuclear transcription factor，NF）-κB信号通路相关基因的相对表达水平。结果：与模型组相比，动物双歧杆菌乳亚种XLTG11能够显著提高DSS诱导溃疡性结肠炎小鼠的体质量变化率（P＜0.05）、结肠长度（P＜0.01），极显著降低DAI、MPO活力和促炎细胞因子质量浓度（P＜0.01），降低致病菌相对丰度和提高产短链脂肪防酸肠道菌群相对丰度，导致短链脂肪酸含量极显著增加（P＜0.01），显著提高肠道屏障相关基因的表达（P＜0.01），抑制核转录因子NF-κB信号通路的激活。结论：动物双歧杆菌乳亚种XLTG11可以提高DSS诱导溃疡性结肠炎小鼠的体质量变化率、结肠长度，降低DAI、MPO活力和促炎细胞因子水平，调节肠道菌群组成，提高短链脂肪酸含量，提高肠道屏障相关基因的表达并抑制NF-κB信号通路的激活，进而有效地缓解DSS诱导的小鼠溃疡性结肠炎。

关键词: 动物双歧杆菌乳亚种；溃疡性结肠炎；肠道菌群；短链脂肪酸；核转录因子信号通路

Abstract: Objective: To investigate the relieving effect of Bifidobacterium animalis subsp. lactis XLTG11 on ulcerative colitis induced by dextran sulfate sodium (DSS) in mice. Methods: Forty-five eight-week-old C57BL/6N male mice were randomly divided into three groups: normal, model and bifidobacterium treatment. To induce ulcerative colitis, the mice were allowed free access to 3% DSS in water. B. animalis subsp. lactis XLTG11 was gavaged to the animals at a dose of 1 × 107 CFU/d. The percentage change of body mass, colon length, disease activity index (DAI) and intestinal myeloperoxidase (MPO) activity were measured. Hstopathological changes were observed in the colon. Tumor necrosis factor (TNF)-α, interleukin 6 (IL-6), IL-1β, and IL-10 contents in the colon tissue were determined. The gut microbiota composition and the contents of fecal short-chain fatty acids were evaluated. The relative expression levels of genes related to intestinal barrier and the nuclear transcription factor (NF)-κB signaling pathway were detected. Results: Compared to the model group, B. animalis subsp. lactis XLTG11 significantly increased the percentage change of body mass (P < 0.05) and colon length (P < 0.01), and decreased DAI, MPO activity and proinflammatory cytokine levels (P < 0.01) in mice with DSS-induced ulcerative colitis. Moreover, it decreased the relative abundance of pathogenic bacteria, increased the relative abundance of short-chain fat acid-producing bacteria in the gut, significantly increased the contents of short-chain fat acids and the expression of genes related to intestinal barrier function (P < 0.01), and inhibited the activation of the NF-κB signaling pathway. Conclusion: B. animalis subsp. lactis XLTG11 could increase the percentage change of body mass and colon length, decrease DAI, MPO activity, and proinflammatory cytokine levels, regulate the gut microbiota composition, increase fecal short-chain fatty acid contents and the expression of intestinal barrier-related genes and inhibit the NF-κB signaling pathway, thereby effectively relieving DSS-induced ulcerative colitis in mice.

Key words: Bifidobacterium animalis subsp. lactis; ulcerative colitis; gut microbiota; short-chain fatty acids; nuclear transcription factor signaling pathway

中图分类号:

TS201.3

马岩，王中江，杨靖瑜，李哲，彭霞，陈昱凤，李柏良. 动物双歧杆菌乳亚种XLTG11对葡聚糖硫酸钠诱导小鼠溃疡性结肠炎的缓解作用[J]. 食品科学, 2022, 43(17): 164-173.

MA Yan, WANG Zhongjiang, YANG Jingyu, LI Zhe, PENG Xia, CHEN Yufeng, LI Bailiang. Alleviating Effect of Bifidobacterium animalis subsp. lactis XLTG11 on Ulcerative Colitis Induced by Sodium Dextran Sulfate in Mice[J]. FOOD SCIENCE, 2022, 43(17): 164-173.

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动物双歧杆菌乳亚种XLTG11对葡聚糖硫酸钠诱导小鼠溃疡性结肠炎的缓解作用

Alleviating Effect of Bifidobacterium animalis subsp. lactis XLTG11 on Ulcerative Colitis Induced by Sodium Dextran Sulfate in Mice

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