低场核磁共振法测定猪肉中脂肪含量

doi:10.7506/spkx1002-6630-20220226-228

食品科学 ›› 2022, Vol. 43 ›› Issue (20): 269-274.doi: 10.7506/spkx1002-6630-20220226-228

低场核磁共振法测定猪肉中脂肪含量

汤舒越，汤晓艳，张宇慧，刘素可，黄欣媛，段胜男

（1.南京农业大学食品科技学院，肉品加工与质量控制教育部重点实验室，江苏南京 210095；2.中国农业科学院农业质量标准与检测技术研究所，农业农村部农产品质量安全重点实验室，北京 100081）

出版日期:2022-10-25 发布日期:2022-10-26
基金资助:
财政部和农业农村部：国家现代农业产业技术体系资助项目；中国农业科学院科技创新工程项目

Determination of Fat Content in Pork by Low-Field Nuclear Magnetic Resonance

TANG Shuyue, TANG Xiaoyan, ZHANG Yuhui, LIU Suke, HUANG Xinyuan, DUAN Shengnan

(1. Key Laboratory of Meat Processing and Quality Control, Collage of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; 2. Key Laboratory of Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs, Institute of Quality Standard and Testing Technology for Agro-products, Chinese Academy of Agricultural Sciences, Beijing 100081, China)

Online:2022-10-25 Published:2022-10-26

摘要/Abstract

摘要： 为避免索氏抽提法使用有机溶剂带来的环境污染与危害，提高猪肉中脂肪含量的检测效率，采用低场核磁共振技术研究测定猪肉中脂肪含量。首先优化回波时间、重复扫描次数等信号采集参数和样品质量、烘干时间、检测温度等样品相关参数，然后对方法重复性和精密度进行考察验证。结果表明，4 g肉糜烘干6 h，在检测温度50 ℃、回波时间0.3 ms、重复扫描次数64 次条件下，以纯猪油为标准样品，检测结果良好，标准曲线R2达到0.999 9，重复性相对标准偏差为1.69%～2.72%，日内、日间精密度分别为3.07%、2.57%。该低场核磁法与GB 5009.6—2016《食品中脂肪的测定》中索氏抽提法之间相关系数高达0.999 5。因此，该方法可用于猪肉中脂肪含量的准确定量，同时可为其他畜禽肉脂肪含量的测定提供参考。

关键词: 低场核磁共振；脂肪含量；烘干；参数优化；弛豫特性

Abstract: In order to avoid environmental pollution and health hazards caused by the use of organic solvents in Soxhlet extraction, and improve the detection efficiency of fat content in pork, a method for determining fat content in pork based on low-field nuclear magnetic resonance (LF-NMR) was proposed. Signal acquisition parameters (echo time and number of scanning cycles), sample mass, drying time and detection temperature were optimized, and the repeatability and precision of the method were verified. When 4 g of minced pork was dried for 6 h in an oven and detected under the following conditions: temperature, 50 ℃; echo time, 0.3 ms; and number scanning cycles, 64, good results were obtained using lard as a standard sample. The correlation coefficient (R2) of the standard calibration curve established was 0.999 9. The repeatability relative standard deviation (RSD) of the proposed method was 1.69%–2.72%, and the intra-day and intra-day precision (RSDs) were 3.07% and 2.57%, respectively. The correlation coefficient between the LF-NMR method and the Soxhlet extraction method specified in the national standard (GB 5009.6–2016) was 0.999 5. Therefore, the LF-NMR method can accurately determine fat content in pork and other meats.

Key words: low-field nuclear magnetic resonance; fat content; drying; parameter optimization; relaxation characteristics

中图分类号:

TS251.7

汤舒越，汤晓艳，张宇慧，刘素可，黄欣媛，段胜男. 低场核磁共振法测定猪肉中脂肪含量[J]. 食品科学, 2022, 43(20): 269-274.

TANG Shuyue, TANG Xiaoyan, ZHANG Yuhui, LIU Suke, HUANG Xinyuan, DUAN Shengnan. Determination of Fat Content in Pork by Low-Field Nuclear Magnetic Resonance[J]. FOOD SCIENCE, 2022, 43(20): 269-274.

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低场核磁共振法测定猪肉中脂肪含量

Determination of Fat Content in Pork by Low-Field Nuclear Magnetic Resonance

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