采后不同温度预贮对雾化栽培微型马铃薯的愈伤效果及其机理

doi:10.7506/spkx1002-6630-20211108-084

食品科学 ›› 2022, Vol. 43 ›› Issue (21): 297-304.doi: 10.7506/spkx1002-6630-20211108-084

• 包装贮运 • 上一篇

采后不同温度预贮对雾化栽培微型马铃薯的愈伤效果及其机理

张志鹏，谭芸秀，李宝军，李永才，毕阳，李守强，王小晶，张宇，胡丹

（1.甘肃农业大学食品科学与工程学院，甘肃兰州 730070；2.甘肃省农业科学院农产品贮藏加工研究所，甘肃兰州 730070；3.甘肃省种子总站，甘肃兰州 730070）

发布日期:2022-12-12
基金资助:
甘肃省重点研发计划项目（GNKJ-2020-2）；国家自然科学基金地区科学基金项目（31860459）；财政部和农业农村部：国家现代农业产业技术体系资助（CARS-09-P26）

Effect and Possible Mechanism of Postharvest Pre-storage Temperature on Wound Healing of Potato Minitubers Grown in Aeroponic System

ZHANG Zhipeng, TAN Yunxiu, LI Baojun, LI Yongcai, BI Yang, LI Shouqiang, WANG Xiaojing, ZHANG Yu, HU Dan

(1. College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China; 2. Institute of Agricultural Products Storage and Processing, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China; 3. Gansu Seed Station, Lanzhou 730070, China)

Published:2022-12-12

摘要/Abstract

摘要： 研究采后不同温度预贮对雾化栽培（以下简称雾培）微型马铃薯愈伤的影响，并进一步探讨其促进愈伤的可能机理。以‘通薯1号’雾培微型马铃薯为试材，在无损伤的条件下，分别于4、15 ℃和25 ℃（相对湿度86%～88%）黑暗环境中预贮20 d。测定预贮期间块茎的质量损失率，观察块茎周皮细胞中软木脂（包括聚酚软木脂（suberin polyphenolics，SPP）和聚酯软木脂（suberin polyaliphatics，SPA））的积累及木栓化情况，分析块茎周皮组织苯丙烷和活性氧代谢的变化。结果表明，15 ℃预贮显著促进了周皮组织处SPP、SPA的积累和木栓化。预贮第5天，与4、25 ℃预贮相比，15 ℃预贮块茎周皮组织处SPP细胞层厚度分别显著增加50.90%和26.94%（P＜0.05），SPA细胞层厚度分别显著增加44.89%和30.23%（P＜0.05），木栓化细胞层厚度分别显著增加27.66%和8.18%（P＜0.05）。相同预贮时间，15 ℃预贮显著提高了块茎周皮组织苯丙氨酸解氨酶（phenylalanine ammonia lyase，PAL）的活力（P＜0.05），增加了总酚、类黄酮和木质素的含量。第5天时，15 ℃预贮块茎PAL活力较4 ℃和25 ℃分别显著增加了34.46%和12.64%（P＜0.05）；总酚和类黄酮含量较4 ℃预贮分别增加11.93%和40.05%，较25 ℃预贮分别增加2.72%和15.50%。第20天时，15 ℃预贮块茎木质素含量较4 ℃和25 ℃分别显著增加24.50%和8.24%（P＜0.05）。此外，相同预贮时间下，15 ℃预贮明显增加了块茎周皮组织处H2O2、超氧阴离子的含量以及过氧化物酶（peroxidase，POD）活力。综上，采后15 ℃预贮可通过激活周皮组织苯丙烷和活性氧代谢，增强周皮组织处软木脂的积累和木栓化，进而加速雾培微型马铃薯的愈伤。

关键词: 微型马铃薯；雾化栽培；温度；愈伤；苯丙烷代谢；活性氧代谢

Abstract: The effect of postharvest pre-storage temperature on the wound healing of potato minitubers grown in an aeroponic system was evaluated, and its possible mechanisms were also explored. Potato mini-tubers (cv. Tongshu 1’) free from artificial damage were pre-stored at 4, 15 or 25 ℃ (86%–88% relative humidity) in a dark environment for 20 days. Mass loss rate was measured during pre-storage, the accumulation of suberin and cork in periderm cells was observed, the degree of suberization was examined, and the changes in phenylpropane and reactive oxygen species metabolism in potato tuber periderm were also analyzed. Results showed that pre-storage at 15 ℃ significantly promoted the accumulation of suberin polyphenolics (SPP) and suberin polyaliphatics (SPA) and the degree of suberization in periderm tissue. On the fifth day of pre-storage, the accumulation of SPP and SPA and the degree of suberization in potato tubers prestored at 15 ℃ were higher than those at 4 and 25 ℃ by 50.90% and 26.94% (P < 0.05); 44.89% and 30.23% (P < 0.05); and 27.66% and 8.18% (P < 0.05), respectively. Pre-storage at 15 ℃ also significantly increased phenylalanine ammonia lyase (PAL) activity (P < 0.05), and augmented the contents of total phenols, flavonoids and lignin. On day 5, PAL activity in potato tubers pre-stored at 15 ℃ was 34.46% and 12.64% higher than that at 4 and 25 ℃, respectively (P < 0.05). The contents of total phenols and flavonoids were 11.93% and 40.05% higher than those at 4 ℃, and 2.72% and 15.50% higher than those at 25 ℃, respectively. On the 20th day, the content of lignin in potato tubers prestored at 15 ℃ was 24.50% and 8.24% higher than that at 4 and 25 ℃, respectively (P < 0.05). In addition, pre-storage at 15 ℃ significantly increased the contents of H2O2 and superoxide anion and peroxidase (POD) activity in periderm tissue. The findings suggested that postharvest pre-storage at 15 ℃ can accelerate the wound healing of potato minitubers grown in an aeroponic system through activating phenylpropane and reactive oxygen species metabolism in periderm tissue, and increasing the accumulation of SPP and SPA and the degree of suberization.

Key words: potato minitubers; aeroponic culture; temperature; wound healing; phenylpropane metabolism; reactive oxygen species metabolism

中图分类号:

TS205.7

张志鹏，谭芸秀，李宝军，李永才，毕阳，李守强，王小晶，张宇，胡丹. 采后不同温度预贮对雾化栽培微型马铃薯的愈伤效果及其机理[J]. 食品科学, 2022, 43(21): 297-304.

ZHANG Zhipeng, TAN Yunxiu, LI Baojun, LI Yongcai, BI Yang, LI Shouqiang, WANG Xiaojing, ZHANG Yu, HU Dan. Effect and Possible Mechanism of Postharvest Pre-storage Temperature on Wound Healing of Potato Minitubers Grown in Aeroponic System[J]. FOOD SCIENCE, 2022, 43(21): 297-304.

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采后不同温度预贮对雾化栽培微型马铃薯的愈伤效果及其机理

Effect and Possible Mechanism of Postharvest Pre-storage Temperature on Wound Healing of Potato Minitubers Grown in Aeroponic System

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