关键词: 鱼腥草；葡聚糖硫酸钠；溃疡性结肠炎；炎症因子

Abstract: The purpose of this study was to investigate the effect of Houttuynia cordata on inflammation and pathological injury in mice with ulcerative colitis (UC) induced by dextran sulfate sodium (DSS). Seventy-two male C57BL/6J mice were randomly divided into blank, model, positive control, Houttuynia cordata juice low-dose, medium-dose and high-dose groups. All groups except the blank one were induced for UC by using 3% DSS solution. Four days later, the animals were intragastrically administered with physical saline, mesalazine or Houttuynia cordata juice (HCJ) for 10 consecutive days. Disease activity index (DAI) was calculated and the levels of malondialdehyde (MDA), tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), interleukin (IL)-1β, IL-4, IL-6, IL-10, myeloperoxidase (MPO) activity and superoxide dismutase (SOD) activity in the colon tissues of mice, and serum lipopolysaccharides (LPS) and D-lactic acid (D-La) levels were measured. Hematoxylin-eosin (HE) staining was used to evaluate histopathological changes in the colon. The results showed that compared with the model group, hematochezia in the low-, medium- and high-dose HCJ groups was improved, and the mental status was better. The body mass, colon length and thymus index of mice in the medium- and high-dose HCJ groups were significantly increased (P < 0.01), DAI, spleen index and the levels of TNF-α, IL-1β, IL-6 and IFN-γ were significantly decreased (P < 0.01), the levels of IL-4 and IL-10 were significantly increased (P < 0.01), the content of MDA and the activity of MPO were significantly decreased (P < 0.01), the activity of SOD was significantly increased (P < 0.01), serum D-La and LPS levels were significantly decreased (P < 0.01), colon tissue injury was significantly reduced, and pathological score was significantly decreased (P < 0.01). In conclusion, Houttuynia cordata juice can improve the inflammatory response of mice with DSS-induced UC, reduce the intestinal permeability, regulate the level of oxidative stress and alleviate intestinal injury.

Key words: Houttuynia cordata; dextran sodium sulfate; ulcerative colitis; inflammatory factor