双频超声联合柠檬酸处理对大黄鱼冷藏期间品质变化的影响

doi:10.7506/spkx1002-6630-20220204-006

食品科学 ›› 2023, Vol. 44 ›› Issue (1): 246-255.doi: 10.7506/spkx1002-6630-20220204-006

双频超声联合柠檬酸处理对大黄鱼冷藏期间品质变化的影响

朱圣赟，蓝蔚青，浦天霆，赵欣宇，徐振飞，谢晶

（1.上海海洋大学食品学院，上海 201306；2.上海水产品加工及贮藏工程技术研究中心，食品科学与工程国家级实验教学示范中心（上海海洋大学），上海 201306）

出版日期:2023-01-15 发布日期:2023-01-31
基金资助:
财政部和农业农村部：国家现代农业产业技术体系资助项目（CARS-47）； “十三五”国家重点研发计划重点专项（2019YFD0901602）；上海水产品加工及贮藏工程技术研究中心能力提升项目（19DZ2284000）

Effect of Dual-Frequency Ultrasound Combined with Citric Acid Treatment on Quality Changes of Large Yellow Croaker (Pseudosciaena crocea) during Refrigerated Storage

ZHU Shengyun, LAN Weiqing, PU Tianting, ZHAO Xinyu, XU Zhenfei, XIE Jing

(1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; 2. Shanghai Aquatic Products Processing and Storage Engineering Technology Research Center, National Experimental Teaching Demonstration Center for Food Science and Engineering, Shanghai Ocean University, Shanghai 201306, China)

Online:2023-01-15 Published:2023-01-31

摘要/Abstract

摘要： 为探究双频超声（dual-frequency ultrasound，DUS）和柠檬酸（citric acid，CA）联合处理对大黄鱼冷藏期间品质变化的影响，分别采用无菌蒸馏水（CK组），20/28 kHz、175 W DUS（DUS组），质量浓度8.0 g/L CA（CA组），DUS联合CA（DUS＋CA组）处理新鲜大黄鱼片10 min。将4 组样品处理后沥干，放入无菌袋中置于4 ℃条件下冷藏，每2 d进行微生物（菌落总数、嗜冷菌数）、理化（质构分析、色差、pH值、总挥发性盐基氮含量、硫代巴比妥酸反应物值）和水分迁移（持水力、低场核磁共振、核磁共振成像）指标测定，并结合感官评价，综合分析不同处理方式对大黄鱼贮藏期间的作用效果。结果表明，CA处理可有效抑制样品冷藏期间的微生物增长和脂肪氧化，CK组样品的菌落总数对数值在第8天时达到（8.26±0.02）（lg（CFU/g）），超过腐败限值，而CA组样品菌落总数对数值为（6.60±0.06）（lg（CFU/g））；此时CA组样品的硫代巴比妥酸值为（0.51±0.05）mg/kg，显著低于对照组（P＜0.05）。DUS组延缓了样品贮藏期间不易流动水（T22）向自由水（T23）的迁移，明显改善了样品的保水性。经DUS＋CA组联合处理后，大黄鱼总体品质优于其他组。贮藏末期，DUS＋CA组样品的总挥发性盐基氮含量为（13.79±0.57）mg/100 g，品质最佳。综合各项指标得出，20/28 kHz、175 W DUS联合质量浓度8.0 g/L CA处理可明显延缓大黄鱼贮藏期间的品质劣变，与CK组对比，其可至少延长大黄鱼冷藏货架期6 d。

关键词: 大黄鱼；冷藏；双频超声；柠檬酸；组织品质

Abstract: In order to explore the combined effects of dual-frequency ultrasound (DUS) and citric acid (CA) on the quality changes of large yellow croaker (Pseudosciaena crocea) during refrigerated storage, fresh fillet samples were treated with sterile distilled water (as a control), DUS at 20/28 kHz and 175 W, 8.0 g/L CA or DUS combined with CA (DUS + CA) for 10 min, packaged in sterile bags and stored at 4 ℃. Microbial parameters (total viable count (TVC), and psychrophilic bacterial count (PBC)), physicochemical properties (texture characteristics, color difference, pH, total volatile base nitrogen (TVB-N) content, thiobarbituric acid reaction substance (TBARS) value), water migration (water-holding capacity (WHC), low-field nuclear magnetic resonance (LF-NMR), magnetic resonance imaging (MRI)) and sensory evaluation were measured every two days during storage. The results showed that CA treatment could inhibit lipid oxidation and microbial growth in large yellow croaker during refrigerated storage. On the 8th day of storage, the logarithm of TVC in the control group was (8.26 ± 0.02) (lg(CFU/g)), which exceeded the spoilage limit and was significantly higher than that of the CA group (6.60 ± 0.06) (lg(CFU/g)). The TBARS value of the CA group was (0.51 ± 0.05) mg/kg on the 8th day of storage, which was significantly lower than that of the control group (P < 0.05). DUS treatment delayed the transformation of immobile water (T22) into free water (T23), indicating improved WHC. Overall, the quality of large yellow croaker treated with DUS + CA was better than that of all other groups during refrigerated storage. At the end of storage, the TVB-N content of the DUS + CA group was (13.79 ± 0.57) mg/100 g and its quality was the best. Collectively, we concluded that 20/28 kHz, 175 W dual-frequency ultrasound combined with 8.0 g/L citric acid treatment can significantly delay the quality deterioration of large yellow croaker, and prolong the shelf-life for at least six days during refrigerated storage compared with the control group.

Key words: Pseudosciaena crocea; refrigerated storage; dual-frequency ultrasound; citric acid; quality

中图分类号:

S983
TS254.4

朱圣赟，蓝蔚青，浦天霆，赵欣宇，徐振飞，谢晶. 双频超声联合柠檬酸处理对大黄鱼冷藏期间品质变化的影响[J]. 食品科学, 2023, 44(1): 246-255.

ZHU Shengyun, LAN Weiqing, PU Tianting, ZHAO Xinyu, XU Zhenfei, XIE Jing. Effect of Dual-Frequency Ultrasound Combined with Citric Acid Treatment on Quality Changes of Large Yellow Croaker (Pseudosciaena crocea) during Refrigerated Storage[J]. FOOD SCIENCE, 2023, 44(1): 246-255.

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双频超声联合柠檬酸处理对大黄鱼冷藏期间品质变化的影响

Effect of Dual-Frequency Ultrasound Combined with Citric Acid Treatment on Quality Changes of Large Yellow Croaker (Pseudosciaena crocea) during Refrigerated Storage

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