油茶籽乙醇提取物代谢物组成及其抗炎活性

doi:10.7506/spkx1002-6630-20220323-280

食品科学 ›› 2023, Vol. 44 ›› Issue (2): 304-311.doi: 10.7506/spkx1002-6630-20220323-280

• 成分分析 • 上一篇

油茶籽乙醇提取物代谢物组成及其抗炎活性

林欣，李杨，詹淼，付湘晋，钟海雁，姚文，刘成

（1.中南林业科技大学食品科学与工程学院，湖南长沙 410004；2.湖南省营养健康品工程技术研究中心，湖南省药食同源功能性食品工程技术研究中心，湖南省调味料绿色制造工程技术研究中心，湖南长沙 410004；3.湖南巨雄油茶油脂研究院，湖南岳阳 414000；4.亿丰农林牧科技有限公司，湖南邵阳 422300）

发布日期:2023-01-31
基金资助:
湖南省重点研发计划项目（2022NK2032）；湖南省教育厅重点项目（18A155）；长沙市重大专项（kh2003013）

Metabolite Composition and Anti-Inflammatory Activity of Ethanol Extract of Camellia oleifera Seed

LIN Xin, LI Yang, ZHAN Miao, FU Xiangjin, ZHONG Haiyan, YAO Wen, LIU Cheng

(1. College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China; 2. Hunan Engineering and Technology Research Center for Nutrition and Health Products, Hunan Engineering Research Center of Functional Food with Homology of Medicine and Food, Hunan Province Seasonings Green Manufacturing Engineering Technology Research Center, Changsha 410004, China; 3. Hunan Ju Xiong Institute of Oil-Tea Camellia Oil, Yueyang 414000, China; 4. Yifeng Agriculture and forestry Co. Ltd., Shaoyang 422300, China)

Published:2023-01-31

摘要/Abstract

摘要： 为探究油茶籽油抗炎机制，采用超高效液相色谱-串联质谱技术检测分析了15 个批次油茶籽乙醇提取物中所有小分子代谢物，并通过“蓝精灵”实验评估油茶籽乙醇提取物对果蝇肠炎的抑制活性，将油茶籽乙醇提取物中小分子代谢物与抗炎活性进行相关性分析并与脂氧合酶进行分子对接，筛选鉴定其关键代谢产物，最后通过“蓝精灵”实验进行验证。结果表明：油茶籽乙醇提取物具有良好的果蝇肠炎抑制活性，抑制率为15.06%～61.71%，其代谢物主要包括多酚类、氨基酸类、有机酸类、核苷酸类、糖类、脂类等，发现7 种多酚类化合物以前未在油茶籽中报道过。初步筛选出具有良好抗肠炎活性的代谢物为：鸦胆子素B、丹参新酮、8-香叶草氧基补骨脂素、异芒果苷、5,7,4’-三羟基-6-异戊烯基异黄酮、山柰苷和山柰酚-3-O-芸香糖苷。5,7,4’-三羟基-6-异戊烯基异黄酮是首次被证实具有抗炎活性，0.1 mg/mL给药量时，果蝇肠炎抑制率为64%，远大于阳性对照柳氮磺胺吡啶（27.99%）。本研究为进一步探究油茶籽提取物内外抗肠炎活性及其机制奠定了基础。

关键词: 油茶籽；抗炎活性；超高效液相色谱-串联质谱；代谢物；分子对接

Abstract: To explore the anti-inflammatory mechanism of Camellia oleifera seed oil, the metabolites in ethanol extracts from 15 batches of C. oleifera seeds were analyzed by ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) and the inhibitory activity of the ethanol extracts on enteritis in Drosophila was evaluated by the “Smurf” test. The correlation between small molecular metabolites in the ethanol extracts and their anti-inflammatory activity was investigated and molecular docking with lipoxidase (LOX) was conducted to identify the key metabolites. Finally, the key metabolites were verified by the “Smurf” test. The results showed that the ethanol extract of C. oleifera seeds had good enteritis inhibitory activity, with percentage inhibition of 15.06%–61.71%, and its metabolites mainly included polyphenols, amino acids, organic acids, nucleotides, sugars and lipids. Seven polyphenolic compounds were identified in C. oleifera seeds for the first time. The monomeric compounds with good anti-enteritis activity were preliminarily identified as bruceine B, miltirone, 8-geranyloxypsoralen, cedrelone, wighteone, kaempferitrin and kaempferol-3-O-rutinoside. The anti-inflammatory activity of wighteone was reported for the first time in this study, and at 0.1 mg/mL, the percentage inhibition of enteritis was 64%, much greater than the positive control sulfasalazine (27.99%). This study lays the foundation for further exploring the anti-enteritis activity and mechanism of C. oleifera seed oil in vitro and in vivo.

Key words: Camellia oleifera seed; anti-inflammatory activity; ultra-high performance liquid chromatography-tandem mass spectrometry; metabolites; molecular docking

中图分类号:

TS221

林欣，李杨，詹淼，付湘晋，钟海雁，姚文，刘成. 油茶籽乙醇提取物代谢物组成及其抗炎活性[J]. 食品科学, 2023, 44(2): 304-311.

LIN Xin, LI Yang, ZHAN Miao, FU Xiangjin, ZHONG Haiyan, YAO Wen, LIU Cheng. Metabolite Composition and Anti-Inflammatory Activity of Ethanol Extract of Camellia oleifera Seed[J]. FOOD SCIENCE, 2023, 44(2): 304-311.

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油茶籽乙醇提取物代谢物组成及其抗炎活性

Metabolite Composition and Anti-Inflammatory Activity of Ethanol Extract of Camellia oleifera Seed

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