食品科学 ›› 2023, Vol. 44 ›› Issue (6): 268-276.doi: 10.7506/spkx1002-6630-20220728-315

• 成分分析 • 上一篇    下一篇

GC-MS/O结合香气活性值表征烧麦中关键香气成分及其贮存过程中的变化情况

刘斌善,魏晓明,邵丹青,董志忠,刘玉平   

  1. (1.北京工商大学轻工科学技术学院,北京 100048;2.中粮营养健康研究院有限公司,北京 102209)
  • 出版日期:2023-03-27 发布日期:2023-03-27
  • 基金资助:
    国家科技计划项目申报中心重点研发计划(子课题)(2017YFD0400501)

Characterization of the Key Odorants in Shaomai and Their Changes during Storage by Gas Chromatography-Mass Spectrometry/Olfactometry Coupled with Odor Activity Value Calculation

LIU Binshan, WEI Xiaoming, SHAO Danqing, DONG Zhizhong, LIU Yuping   

  1. (1. School of Light Industry, Beijing Technology and Business University, Beijing 100048, China; 2. COFCO Nutrition & Health Research Institute Co. Ltd., Beijing 102209, China)
  • Online:2023-03-27 Published:2023-03-27

摘要: 为确定烧麦中的关键香气成分及其贮存过程中的变化情况,采用溶剂萃取结合溶剂辅助风味成分蒸发提取烧麦中的挥发性成分,所得提取物经气相色谱-嗅闻联用仪与气相色谱-质谱联用仪分析,共鉴定出50 个香气活性成分。通过香气提取物稀释分析,测定香气活性成分的香气稀释因子,其中1-辛烯-3-醇、茴香脑、愈创木酚、2-戊基呋喃等具有最高的香气稀释因子(6 561)。采用内标标准曲线法对37 个香气稀释因子不低于81的成分进行定量分析;根据定量结果和阈值,计算物质的香气活性值,其中21 个香气活性成分的香气活性值不低于1,被确定为烧麦的关键香气成分。通过分析室温贮存0~13 个月后烧麦中37 个成分含量并计算其香气活性值,发现烧麦贮存过程中关键香气成分的数量不变,除酱油酮、己醛和4-乙烯基愈创木酚的含量下降外,烧麦中多数重要香气活性成分含量变化较小。

关键词: 烧麦;香气活性成分;香气提取物稀释分析;香气活性值;关键香气成分;贮存

Abstract: In order to determine the key odorants in Shaomai (SM) and to investigate the changes of the odorants during SM storage, volatiles were extracted by solvent extraction combined with solvent-assisted flavor evaporation. A total of 50 odor-active compounds were identified by gas chromatography-olfactometry (GC-O) and gas chromatography-mass spectrometry (GC-MS). Among these compounds, 1-octen-3-ol, anethole, guaiacol and 2-pentylfuran had the highest flavor dilution (FD) of 6 561 as determined by aroma extract dilution analysis (AEDA). Thirty-seven compounds with FD factors ≥ 81 were quantitated by the internal standard method. Based on the quantitative results and odor thresholds reported in the literature, their odor activity values (OAVs) were calculated. Totally 21 odorants with OAV ≥ 1 were determined as the key odorants of SM. The contents of the 37 compounds in SM stored for up to 13 months at room temperature were analyzed and their OAVs were calculated. The results showed that the number of key odorants in SM did not change during storage. The levels of 5-ethyl-4-hydroxy-2-methyl-3(2H)-furanone, hexanal and 4-vinylguaiacol decreased greatly, while most of the other compounds changed little.

Key words: Shaomai; odor active compounds; aroma extract dilution analysis; odor activity value; key odorants; storage

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