不同松花粉提取物对环磷酰胺诱导大鼠卵巢损伤的保护作用

摘要/Abstract

摘要： 环磷酰胺是临床上针对多种肿瘤及自身免疫性疾病应用较为广泛的一种烷化剂药物，会造成患者卵巢损伤，严重至不育及危害终身健康。松花粉作为我国传统的药食两用物质，其全粉已被证实具有对环磷酰胺诱导的卵巢损伤的保护作用。然而，松花粉中对卵巢损伤起到保护作用的主要成分尚不明确。本文通过制备松花粉水提物、水提大分子化合物（> 5 kDa）、乙醇提物和乙醇提小分子化合物（< 5 kDa），测定了各组提取物中的总糖、粗多糖、总酚和总黄酮含量，并考察了其对摄入环磷酰胺的SD大鼠的卵巢保护作用。结果表明，相对模型组，松花粉乙醇提物及乙醇提小分子化合物组有效提升了卵巢指数（分别相对提升了34.94%和33.70%）、成熟卵泡数量、卵巢抗氧化酶活力（T-AOC、CAT、GSH-Px和SOD分别相对提升了16.03%、25.81%，138.42%、237.09% ，104.31%、109.90%和91.62%、120.63%）和抗细胞凋亡蛋白表达，及抑制闭锁卵泡和卵巢脂质氧化（MDA分别相对降低了53.42%和48.43%）等，效果优于松花粉水提物及水提大分子化合物组，但松花粉水提物及水提大分子化合物可以通过降低促炎因子TNF-α（分别相对降低了61.73%和61.11%）保护卵巢。本研究可为进一步探明松花粉的卵巢保护作用机制提供理论基础，为开发基于松花粉提取物的新产品提供技术指导。

关键词: 松花粉, 水提物, 乙醇提物, 水提大分子化合物, 乙醇提小分子化合物, 卵巢损伤

Abstract: Cyclophosphamide is an alkylating agent widely used in clinical practice for a variety of tumors and autoimmune diseases, which can cause ovarian damage to the patient's ovaries, resulting in severe infertility and lifelong health risks. Pine pollen, a traditional dual-use substance in China, has been shown to have a protective effect on ovaries in cyclophosphamide ingested organisms. It is difficult to investigate the ovarian protective mechanism of pine pollen due to the influence of multiple substances contained in it with multi-target and multi-pathway integrated regulation. In this paper, we prepared aqueous extract, large molecule aqueous extract (> 5 kDa), alcoholic extract and small molecule alcoholic extract (< 5 kDa) of pine pollen, and determined the contents of total sugars, crude polysaccharides, total phenols and total flavonoids in each group of extracts and investigated their ovarian protective effects on SD rats ingested with cyclophosphamide. The results showed that relative to the model group, the pine pollen alcoholic extract and alcoholic small molecule group effectively increased the ovarian index (34.94% and 33.70% relative increase, respectively), the number of mature follicles, ovarian antioxidant enzyme activity (T-AOC, CAT, GSH-Px and SOD increased by 16.03%, 25.81%; 138.42%, 237.09%; 104.31%, 109.90%; and 91.62%, 120.63%, respectively) and anti-apoptotic protein expression and inhibition of atretic follicles and ovarian lipid oxidation (MDA was reduced by 53.42% and 48.43%). The effectiveness of pine pollen alcoholic extract and alcoholic small molecule group were better than the aqueous extract of pine pollen and aqueous macromolecule group, but aqueous extract of pine pollen and aqueous macromolecule could reduce the pro-inflammatory factor TNF-α (61.73% and 61.11% relative reduction, respectively) to protect the ovaries. This study provides a theoretical basis to further investigate the mechanism of ovarian protective effect of pine pollen and provides technical guidance for the development of new products based on pine pollen extracts.

Key words: pine pollen, aqueous extracts, alcoholic extracts, aqueous macromolecules, ethanol extracts of small molecules, ovarian injury

中图分类号:

R284.1

刘其耸陈阳杨长军何毓婕陈秋铭何志勇王召君陈洁曾茂茂. 不同松花粉提取物对环磷酰胺诱导大鼠卵巢损伤的保护作用[J]. 食品科学.

Qi-Song LIU Jie Chen Maomao maoZeng. Protective effect of difference pine pollen extracts on ovarian damage in SD rats induced by cyclophosphamide[J]. FOOD SCIENCE.

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