竹荪水提物抗氧化及改善秀丽隐杆线虫脂质代谢作用

doi:10.7506/spkx1002-6630-20211004-024

食品科学 ›› 2022, Vol. 43 ›› Issue (19): 191-199.doi: 10.7506/spkx1002-6630-20211004-024

竹荪水提物抗氧化及改善秀丽隐杆线虫脂质代谢作用

肖嵋方，陈欣彤，蔡雯雯，李娜，陈海明，刘斌，曾峰

（1.福建农林大学食品科学学院，福建福州 350002；2.海南省食品营养与功能食品重点实验室，海南海口 570228；3.福建农林大学国家菌草工程技术研究中心，福建福州 350002）

出版日期:2022-10-15 发布日期:2022-10-26
基金资助:
江西省重点研发计划项目（20192ACB60008）；吉安市重大科技专项（吉财教[2019]55号）；海南省食品营养与功能食品重点实验室开放基金项目（KF202003）

Antioxidant Activity of Water Extract from Dictyophora indusiate and Its Potential to Improve Lipid Metabolism in Caenorhabditis elegans

XIAO Meifang, CHEN Xintong, CAI Wenwen, LI Na, CHEN Haiming, LIU Bin, ZENG Feng

(1. College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2. Hainan Key Laboratory of Food Nutrition and Functional Food, Haikou 570228, China; 3. China National Engineering Research Center of JUNCAO Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China)

Online:2022-10-15 Published:2022-10-26

摘要/Abstract

摘要： 以1,1-二苯基-2-三硝基苯肼（1,1-diphenyl-2-picrylhydrazyl，DPPH）自由基、羟自由基与2,2’-联氨-双(3-乙基苯并噻唑啉-6-磺酸)（2,2’-azino-bis(3-ethylbenzthiazoline-6-sulphonate)，ABTS）阳离子自由基清除能力3 个指标评价竹荪水提物的体外抗氧化能力；以秀丽隐杆线虫（简称线虫）为模型，观察低、中、高剂量（0.25、0.5、1.0 mg/mL）的竹荪水提物对线虫氧化应激与热应激的影响，采用油红O染色法，研究不同质量浓度竹荪水提物对线虫体内脂肪沉积的影响，同时测定了线虫体内甘油三酯浓度和脂肪代谢相关基因的表达水平。结果发现，竹荪水提物对DPPH、羟自由基和ABTS阳离子自由基的半抑制质量浓度分别为2.36、1.44、0.86 mg/mL，显著提高了线虫的抗氧化能力，其中高剂量组使氧化应激和热应激状态下线虫的平均寿命分别延长了54.6%（P＜0.01）和19.8%（P＜0.05）；与空白组相比，竹荪水提物高剂量组线虫体内的脂质沉积水平和甘油三酯浓度分别极显著减少了57.0%和40.5%（P＜0.01），其机制可能是竹荪水提物诱导mod-1、daf-1、fat-7、acs-2、daf-16和mdt-15基因表达水平的下调，以及诱导daf-2、sbp-1、nhr-49、fat-5和fat-6基因表达水平的上调，从而改善线虫脂质代谢。结果表明，竹荪水提物具有抗氧化和改善脂质代谢的功能特性。

关键词: 竹荪水提物；抗氧化；秀丽隐杆线虫；脂质代谢

Abstract: The in vitro antioxidant activity of water extract from Dictyophora indusiate was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonate) (ABTS) cation radical scavenging assays. Caenorhabditis elegans was used as a model organism to observe the effects of the extract at high (1.0 mg/mL), medium (0.5 mg/mL) and low (0.25 mg/mL) doses on oxidative and heat stress. In addition, the effects of the extract on fat deposition was analyzed by oil red O staining, and the level of triglyceride (TG) and the expression of fat metabolism-related genes in C. elegans were determined. The results showed that the extract enhanced antioxidant capacity in C. elegans. Its half maximal inhibitory concentrations on DPPH radical, hydroxyl radical and ABTS cation radical were 2.36, 1.44 and 0.86 mg/mL, respectively. The average life span of C. elegans under oxidative and heat stress was prolonged by 54.6% (P < 0.01) and 19.8% (P < 0.05) in the high-dose group, respectively. Compared with the control group, lipid deposition and TG level in the high-dose group decreased by 57.0% (P < 0.01) and 40.5% (P < 0.01), respectively. The mechanism may be that the water extract can down-regulate the regulation of mod-1, daf-1, fat-7, acs-2, daf-16 and mdt-15, up-regulate the expression levels of daf-2, sbp-1, nhr-49, fat-5 and fat-6, thereby improving lipid metabolism. These results show that the water extract of D. indusiate has antioxidant properties and can improve lipid metabolism.

Key words: water extract from Dictyophora indusiate; antioxidant; Caenorhabditis elegans; lipid metabolism

中图分类号:

TS254.1

肖嵋方，陈欣彤，蔡雯雯，李娜，陈海明，刘斌，曾峰. 竹荪水提物抗氧化及改善秀丽隐杆线虫脂质代谢作用[J]. 食品科学, 2022, 43(19): 191-199.

XIAO Meifang, CHEN Xintong, CAI Wenwen, LI Na, CHEN Haiming, LIU Bin, ZENG Feng. Antioxidant Activity of Water Extract from Dictyophora indusiate and Its Potential to Improve Lipid Metabolism in Caenorhabditis elegans[J]. FOOD SCIENCE, 2022, 43(19): 191-199.

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竹荪水提物抗氧化及改善秀丽隐杆线虫脂质代谢作用

Antioxidant Activity of Water Extract from Dictyophora indusiate and Its Potential to Improve Lipid Metabolism in Caenorhabditis elegans

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