关键词: 原料乳, 冷藏, 蛋白组学, 差异蛋白质, 生物信息学

Abstract: Abstract: Using data independent acquisition (DIA) proteomics, the compositions and biological functions of differentially expressed proteins in raw milk during cold storage at 4 ℃ for 6 days were investigated. A total of 902 proteins were detected. Among them, 70 and 71 differentially expressed proteins were screened during the early stage of refrigeration (d 0 vs d 3) and the late stage of refrigeration (d 3 vs d 6), respectively. The differentially expressed proteins played an important role in the response to nerve growth factors and the regulation of cell tissues in the early stage of refrigeration, which were mainly involved in the biological processes of cells. In the late stage of refrigeration, the differentially expressed proteins could regulated carbohydrate metabolic process and RNA metabolic process, and mainly participated in the sugar metabolic pathways. Protein-protein interaction network (PPI) analysis denoted that the cell division control protein 42 homolog (CDC42) was the hub node protein in both refrigeration periods, which was closely related to phagocytosis. These findings revealed the changes of compositions and the diversity of functions in raw milk protein during refrigeration, and provided a theoretical basis for the cold storage of raw milk, which had an important significance for the quality control of raw.

Key words: raw milk, refrigeration, proteomics, differentially expressed proteins, bioinformatics