基于脂质组学研究胡椒碱对肥胖大鼠脂代谢基因昼夜节律的影响

食品科学

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基于脂质组学研究胡椒碱对肥胖大鼠脂代谢基因昼夜节律的影响

张玮芸¹,Chi-Tang Ho²,吕慕雯¹

1. 华南农业大学食品学院
2. 美国罗格斯大学食品科学系

收稿日期:2023-06-02 修回日期:2023-09-05 出版日期:2023-09-26 发布日期:2023-09-26
通讯作者: 吕慕雯
基金资助:
广州市基础研究计划基础与应用基础研究项目;广东省“珠江人才计划”引进创新创业团队;广东省自然科学基金-面上项目

Effect of piperine on circadian rhythm of lipid metabolism-associated genes in obese rats based on lipidomics analysis

Received:2023-06-02 Revised:2023-09-05 Online:2023-09-26 Published:2023-09-26
Supported by:
the Science and Technology Program of Guangzhou, China;The Program for Guangdong Introducing Innovative and Entrepreneurial Teams;the Natural Science Foundation of Guangdong Province, China

摘要/Abstract

摘要： 作为胡椒中的主要生物活性成分，胡椒碱（1-piperoylpiperidine, PIP）已被证明具有调节脂质代谢，预防肥胖等功效。据研究报道，生物钟系统参与了脂质代谢中合成与分解的关键步骤，维持着机体的脂质稳态，因此调节昼夜节律也成为预防脂质代谢紊乱的重要手段。本研究采用高脂饮食（High fat diet，HFD）诱导的肥胖大鼠模型，探究口服PIP对代谢紊乱引发的生物钟异常的调控作用。结果表明，PIP减轻了HFD诱导的肝脏损伤，降低了血脂水平，缓解了肝脏生物钟和脂代谢基因的昼夜节律紊乱，恢复了与生物钟密切相关的脂质分子溶血磷脂酸（Lysobisphosphatidic acids，LBPA）（16:0_18:1），双甲基磷脂酸（Bis-methyl phosphatidic acid，BisMePA）（38:8e），磷脂酰乙醇胺（Phosphatidyl ethanolamine，PE）（16:0p_22:6）和甲基磷胆碱（Methylphosphocholine，MePC）（32:4e）的表达水平变化。这些发现可为PIP调节肝脏脂代谢昼夜节律提供重要的理论依据。

关键词: 胡椒碱, 肥胖大鼠, 脂质代谢, 昼夜节律

Abstract: As the main bioactive component in pepper, piperine (1-piperoylpiperidine, PIP) has been shown to have a modulating effect on lipid metabolism and obesity. It has been reported that the circadian clock system is involved in the key steps of lipid synthesis and degradation, which maintains lipid homeostasis inside the body. Therefore, regulating the circadian system has become an important strategy in preventing lipid metabolism disorder. In this study, an obese rat model induced by a high-fat diet (HFD) was used to investigate the modulating effect of PIP on circadian misalignment caused by metabolic dysregulation. The results showed that PIP attenuated HFD-induced liver damage and lipid disorders, alleviated the circadian rhythm disruption of hepatic clock genes and lipid metabolism-associated genes, and restored the expression levels of lysobisphosphatidic acids (LBPA) (16:0_18:1), bis-methyl phosphatidic acid (BisMePA) (38:8e), phosphatidyl ethanolamine (PE) (16:0p_22:6) and methylphosphocholine (MePC) (32:4e), which are closely associated with circadian rhythm gene expressions. These findings may provide an important theoretical basis for PIP in regulating circadian rhythms of hepatic lipid metabolism.

Key words: Piperine, obese rats, lipid metabolism, circadian rhythm

中图分类号:

TS201.4

张玮芸 Chi-Tang Ho 吕慕雯. 基于脂质组学研究胡椒碱对肥胖大鼠脂代谢基因昼夜节律的影响[J]. 食品科学.

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基于脂质组学研究胡椒碱对肥胖大鼠脂代谢基因昼夜节律的影响

Effect of piperine on circadian rhythm of lipid metabolism-associated genes in obese rats based on lipidomics analysis

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