短期服用蛹虫草对不同状态小鼠的免疫调节作用

摘要/Abstract

摘要： 目的：旨在研究短期服用蛹虫草对不同状态小鼠的免疫调节作用，探讨其作为免疫调节剂的应用价值。方法：KM系小鼠随机分为低倍组（DB组）、高倍组（GB组）和对照组（CK组），DB组和GB组连续灌胃不同剂量蛹虫草水溶液 21 d，0.5 mL/只，CK组为同等剂量蒸馏水。测定小鼠脏器指数、血清IgG、IgM、IL-2和IL-10含量，外周血CD3+CD4+和CD3+CD8+T淋巴细胞亚群含量、肠道菌群组成结构及空肠TLRs、MYD88及NF-κB表达量的变化。腹腔注射80 mg/kg.bw 环磷酰胺溶液诱导小鼠免疫抑制状态，蛹虫草水溶液灌胃的第0 d、7 d、14 d、21 d开展取材检测。结果：(1)不同剂量蛹虫草对小鼠体重、脏器指数、血清IgG、IgM、 IL-2和IL-10水平、外周血CD3+CD4+和CD3+CD8+T淋巴细胞亚群含量均未产生显著性改变(P>0.05)。(2)蛹虫草可以显著上调小鼠肠道菌群Shannon指数(P<0.05)和OTU 数量，增加肠道菌群多样性和丰富度。肠道益生菌Lactobacillus和Alistipes相对丰度明显升高(P<0.05)，炎症促生菌Clostridium XlVa、Helicobacter及Lachnospiracea-incertae-sedis 丰度显著降低(P<0.05)。(3)不同剂量蛹虫草均引起小鼠空肠组织TLR4表达量的显著降低 (P<0.05)，NF-κB表达量的降低程度与蛹虫草灌胃剂量呈正相关。(4)蛹虫草能够提前恢复环磷酰胺导致的脏器损伤，显著提高免疫抑制小鼠IgM、IgG 和IL-2血清水平(P<0.05)，促进外周血CD3+CD4+和CD3+CD8+T淋巴细胞亚群的增殖(P>0.05) 。结论：短期服用蛹虫草可以降低肠道炎症的发生风险，不会引起正常免疫状态的失衡；蛹虫草可以通过增强体液免疫和细胞免疫促进机体免疫抑制状态的恢复，可用于免疫调节剂的开发和利用。

关键词: 蛹虫草, 免疫调节, 肠道菌群, 免疫抑制

Abstract: Abstract: Objective: To investigate the immunomodulatory effects of short-term administration of Cordyceps militaris on mice in different states, and to explore its application value as an immunomodulator. Methods: KM mice were randomly divided into low dosage group (DB group), high dosage group (GB group) and control group (CK group). The mice in DB and GB groups were continuously gavaged with Cordyceps militaris aqueous solution for 21 days, 0.5 mL/ animal, and CK group were given the same dose of distilled water. After 21 days, the organ index, serum IgG, IgM, IL-2 and IL-10 levels, peripheral blood CD3+CD4+ and CD3+CD8+T lymphocyte subsets, intestinal flora composition and TLRs-MYD88-NF-κB expression in the jejunum were measured. At the same time, Mice were injected with 80 mg/kg.bw cyclophosphamide solution intraperitoneally to induce immunosuppression. Samples were collected and detected on the 0, 7, 14 and 21 days respectively after intragastric administration of Cordyceps militaris aqueous solution. Results: (1) Different doses of Cordyceps militaris did not cause significant changes in body weight, organ index, serum IgG and IgM levels, CD3+CD4+ and CD3+CD8+T lymphocyte subsets in peripheral blood, serum IL-2 and IL-10 levels (P>0.05). (2) Cordyceps militaris up-regulated Shannon index (P<0.05) and OTU number of intestinal flora significantly. It increased the diversity and richness of intestinal flora. The relative abundances of intestinal probiotic Lactobacillus and Alistipes were significantly increased (P<0.05). The abundances of inflammatory growth promoting bacteria Clostridium XlVa, Helicobacter and Lachnospiracea-incertae-sedis were significantly decreased (P<0.05). (3) The expression of TLR4 in jejunum tissues of mice was significantly decreased at different doses of Cordyceps militaris (P<0.05), and the reduction of NF-κB expression was positively correlated with the dosage of Cordyceps militaris. (4) Cordyceps militaris significantly restored the organ damage caused by cyclophosphamide. It increased the serum levels of IgM, IgG and IL-2 (P<0.05), and promoted the proliferation of CD3+CD4+ and CD3+CD8+T lymphocyte subsets in the peripheral blood of immunosuppressed mice (P>0.05). Conclusions: Short-term administration of Cordyceps militaris can reduce the risk of intestinal inflammation. It does not cause the imbalance of normal immune status. Cordyceps militaris can promote the recovery of immunosuppression by enhancing humoral and cellular immunity. It can be used for the development and utilization of immunomodulator.

Key words: Cordyceps militaris, Immune regulation, intestinal flora, Immunosuppression

中图分类号:

R285.5

张美娜赵南南廖思晴王升厚王泽. 短期服用蛹虫草对不同状态小鼠的免疫调节作用[J]. 食品科学.

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