食品科学 ›› 2024, Vol. 45 ›› Issue (17): 253-264.doi: 10.7506/spkx1002-6630-20231214-115

• 专题论述 • 上一篇    下一篇

蛋白酶的固定化及其在食品工业中应用的研究进展

陈尚文, 邓乾春, 周彬, 唐小倩, 彭登峰, 陈亚淑, 邓紫玙   

  1. (1.湖北工业大学生命科学与健康工程学院,湖北 武汉 430068;2.中国农业科学院油料作物研究所,油料脂质化学与营养湖北省重点实验室,湖北 武汉 430062)
  • 出版日期:2024-09-15 发布日期:2024-09-09
  • 基金资助:
    国家自然科学基金青年科学基金项目(32302149);中央级公益性科研院所基本科研业务费专项(1610172023005); 知识创新专项武汉市曙光计划项目(中国农业科学院油料作物研究所2023020201020397)

Research Progress in Immobilization of Protease and Its Application in Food Industry

CHEN Shangwen, DENG Qianchun, ZHOU Bin, TANG Xiaoqian, PENG Dengfeng, CHEN Yashu, DENG Ziyu   

  1. (1. School of Life and Health Sciences, Hubei University of Technology, Wuhan 430068, China; 2. Hubei Key Laboratory of Lipid Chemistry and Nutrition, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China)
  • Online:2024-09-15 Published:2024-09-09

摘要: 蛋白酶在实际生产中有着广泛的应用,但游离的蛋白酶抵抗外界不良环境的能力较差,容易造成活性丧失,且难以回收的缺点在一定程度上限制了其在食品工业中的应用。固定化酶技术可以很好地解决上述问题。本文首先从固定化载体和固定化方法两个角度对固定化蛋白酶的相关研究进行总结,指出其中可能面临的挑战,并提出今后可能的研究方向和趋势。接着从应用的角度,阐述固定化蛋白酶在改善蛋白质功能特性和制备功能活性多肽方面的巨大潜力。因此,将更多形式的固定化蛋白酶应用于食品工业中是今后重要的发展方向之一。

关键词: 蛋白酶;固定化;蛋白质;食品工业;应用;研究进展

Abstract: Protease has a wide range of applications in actual production, but its free form has poor resistance to adverse external environments, readily causing activity losses. Moreover, free protease is difficult to recover, limiting its application in the food industry. Enzyme immobilization technology can solve these problems well. This article summarizes recent research on immobilized protease from two aspects: immobilization carriers and methods, points out possible challenges, and proposes possible future research directions. Subsequently, this review discusses the great potential of immobilized protease in improving the functional properties of proteins and preparing functionally active peptides. Therefore, the application of more forms of immobilized proteases in the food industry is an important development direction in the future.

Key words: protease; immobilization; protein; food industry; application; research progress

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