食品科学 ›› 2024, Vol. 45 ›› Issue (21): 245-253.doi: 10.7506/spkx1002-6630-20240407-048

• 食品工程 • 上一篇    

超声联合酶解技术制备高溶解性蛋黄颗粒粉

于春晖, 李博   

  1. (中国农业大学食品科学与营养工程学院,北京 100083)
  • 发布日期:2024-11-05
  • 基金资助:
    “十四五”国家重点研发计划重点专项(2022YFD2101003)

Ultrasound-Assisted Enzymatic Preparation of Highly Soluble Egg Yolk Granule Powder

YU Chunhui, LI Bo   

  1. (College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China)
  • Published:2024-11-05

摘要: 采用超声联合蛋白酶酶解技术使蛋黄颗粒解聚,并在此基础上制备高溶解性的蛋黄颗粒粉。结果表明:当料液比为1∶12(g/mL)时,超声联合酶解处理后蛋黄颗粒的溶解度从8.1 g/100 g提升至89.7 g/100 g,可溶性蛋白质量浓度提高了1.16 倍,平均粒径从24.74 μm减小到1.05 μm,分散稳定性显著提升。当料液比提升至1∶9,蛋黄颗粒的溶解度仍能达到85.0 g/100 g以上。经灭酶、杀菌后解聚颗粒的溶解度为53.7 g/100 g。蛋黄颗粒粉在应用前经过分散处理,溶解度高达86.5 g/100 g,分散液在80 ℃及以下热处理20 min、体系pH 6.5~7.5、盐添加量2%以内的环境下24 h内均可保持分散稳定性。与蛋黄粉相比,蛋黄颗粒粉具有高蛋白、低脂和低胆固醇的特点,高溶解性蛋黄颗粒粉制备技术为实现蛋黄颗粒粉工业化生产和多场景应用提供技术支撑。

关键词: 超声;酶解;蛋黄颗粒粉;溶解性

Abstract: This study investigated the ultrasound-assisted enzymatic depolymerization of egg yolk granules and the subsequent preparation of highly soluble egg yolk granule powder. The results showed that after ultrasound-assisted enzymatic treatment at solid-to-liquid ratio of 1:12 (g/mL), the solubility of egg yolk granules increased from 8.1 to 89.7 g/100 g, the soluble protein concentration increased by 2.16 times, the average particle size decreased from 24.74 to 1.05 μm, and the dispersion stability increased markedly. When the solid-to-liquid ratio was increased to 1:9, the solubility of egg yolk granules was still as high as over 85.0 g/100 g. The solubility of depolymerized granules was 53.7 g/100 g after enzyme inactivation and sterilization. After dispersion treatment, egg yolk powder had solubility as high as 86.5 g/100 g. After heat treatment at 80 ℃ or below for 20 min, pH adjustment to 6.5–7.5, or addition of salt at levels below 2%, the dispersion solution could remain stable for 24 h. Compared with egg yolk powder, egg yolk granule powder has the characteristics of higher protein, lower fat, and lower cholesterol. The preparation technology of highly soluble egg yolk granule powder provides technical support for the industrial production and multi-scenario application of egg yolk granule powder.

Key words: ultrasound; enzymatic hydrolysis; egg yolk granule powder; solubility

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