基于广泛靶向代谢组学技术的掌叶覆盆子不同部位代谢产物差异分析

doi:10.7506/spkx1002-6630-20250311-093

食品科学 ›› 2025, Vol. 46 ›› Issue (17): 231-219.doi: 10.7506/spkx1002-6630-20250311-093

• 成分分析 • 上一篇

基于广泛靶向代谢组学技术的掌叶覆盆子不同部位代谢产物差异分析

于强，杨敏，潘萍，陈菲，王显湧，邓尚贵，陈宏降，罗益远

（1.浙江海洋大学食品与药学学院，浙江?舟山 316022；2.浙江药科职业大学中药学院，浙江?宁波 315500；3.如皋市中医院药剂科，江苏?如皋 226599）

发布日期:2025-08-18
基金资助:
浙江省公益技术研究计划项目（LTGN23H280004）；浙江省药品监督管理局科技计划项目（2025031）；宁波市自然科学基金项目（2023J306）；浙江药科职业大学校级重点课题（2022128）；南通市卫生健康委科研课题（QNZ2024094）；江苏省药学会——“药”研新声药学科研项目课题（202495038）；如皋市科技攻关计划（农业和社会发展）项目（SRGS（24）013）

Differential Analysis of Metabolites in Different Parts of Rubus chingii Hu Based on Widely Targeted Metabolomics

YU Qiang, YANG Min, PAN Ping, CHEN Fei, WANG Xianyong, DENG Shanggui, CHEN Hongjiang, LUO Yiyuan

(1. Food and Pharmacy College, Zhejiang Ocean University, Zhoushan 316022, China;2. College of Traditional Chinese Medicine, Zhejiang Pharmaceutical University, Ningbo 315500, China;3. Pharmacy Department, Rugao Hospital of Traditional Chinese Medicine, Rugao 226599, China)

Published:2025-08-18

摘要/Abstract

摘要： 为探究掌叶覆盆子不同部位中代谢物组成及差异，采用基于超高效液相色谱-质谱的广泛靶向代谢组学技术对掌叶覆盆子不同部位（根、果、叶、茎）样品的代谢物分析，并比较分析各部位差异。研究结果表明，在掌叶覆盆子的4 个部位中共检测到1 726 种代谢物，并从中筛选出610 个差异代谢物，这些代谢物可分为酸类、萜类、苷类、黄酮类等16 类化合物。通过多元统计分析和聚类热图分析发现，掌叶覆盆子各部位样品显著分离并各自聚为一类，表明不同部位的代谢物存在明显差异。进一步分析各部位间差异代谢物发现，与根、果和茎相比，叶中的差异代谢物表达普遍上调。掌叶覆盆子主要活性成分黄酮类物质在根、果、叶中广泛分布，山柰酚-3-O-芸香糖苷在果中相对含量最高，鞣花酸在根中含量最高；黄酮类代谢物在根中含量丰富，果中富含酚酸类代谢物，叶中萜类、生物碱类、苯丙素类差异代谢物含量丰富，其中咖啡酸、绿原酸、水杨酸等含量最高；茎中没食子酸、儿茶素、表儿茶素、原花青素衍生物等代谢物含量较高。对差异代谢物进行京都基因与基因组百科全书富集分析，代谢物主要注释和富集于类黄酮生物合成、类固醇激素生物合成、花生四烯酸代谢、二萜类生物合成。研究结果揭示了掌叶覆盆子不同部位代谢物特征，可为掌叶覆盆子的精细开发和综合利用以及实现掌叶覆盆子资源的循环利用与绿色发展提供理论参考。

关键词: 掌叶覆盆子；广泛靶向代谢组学；不同部位；超高效液相色谱-质谱；差异代谢物

Abstract: The differences in metabolite composition among different parts (roots, fruits, leaves and stems) of Rubus chingii Hu (R. chingii) were analyzed using widely targeted metabolomics based on ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS). The results showed that a total of 1 726 metabolites were detected, and 610 differential metabolites belonging to 16 classes, such as acids, terpenes, glycosides and flavonoids, were selected between the four parts. Through multivariate statistical analysis and cluster heatmap analysis, it was found that the metabolomes of these four parts were clearly separated from each other, each being clustered into one group. Furthermore, the expression of differential metabolites was generally up-regulated in the leaves compared with the roots, fruits and stems. Flavonoids, the major functional components of R. chingii, were widely distributed in the roots, fruits and leaves. The relative contents of kaempferol-3-O-rutinoside and ellagic acid were the highest in the fruits and roots, respectively. Flavonoid metabolites were abundant in the roots, and the fruits were rich in phenolic acid metabolites. Terpenoids, alkaloids and phenylpropanoids were abundant in the leaves, among which the contents of caffeic acid, chlorogenic acid and salicylic acid were the highest. Gallic acid, catechin, epicatechin and proanthocyanidin derivatives were abundant in the stems. Kyoto Encyclopedia of Genes and Genomes enrichment analysis showed that the differential metabolites were mainly annotated and enriched in flavonoid biosynthesis, steroid hormone biosynthesis, arachidonic acid metabolism, and diterpenoid biosynthesis. The findings provide a theoretical reference for the development and comprehensive utilization of R. chingii, as well as the circular utilization and green development of R. chingii resources.

Key words: Rubus chingii Hu; widely targeted metabolomics; different parts; ultra-high performance liquid chromatography-mass spectrometry; differential metabolites

中图分类号:

TS255.1

于强，杨敏，潘萍，陈菲，王显湧，邓尚贵，陈宏降，罗益远. 基于广泛靶向代谢组学技术的掌叶覆盆子不同部位代谢产物差异分析[J]. 食品科学, 2025, 46(17): 231-219.

YU Qiang, YANG Min, PAN Ping, CHEN Fei, WANG Xianyong, DENG Shanggui, CHEN Hongjiang, LUO Yiyuan. Differential Analysis of Metabolites in Different Parts of Rubus chingii Hu Based on Widely Targeted Metabolomics[J]. FOOD SCIENCE, 2025, 46(17): 231-219.

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基于广泛靶向代谢组学技术的掌叶覆盆子不同部位代谢产物差异分析

Differential Analysis of Metabolites in Different Parts of Rubus chingii Hu Based on Widely Targeted Metabolomics

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