食品科学 ›› 2025, Vol. 46 ›› Issue (18): 92-91.doi: 10.7506/spkx1002-6630-20250121-153

• 生物工程 • 上一篇    下一篇

基于全基因组重测序的赤霞珠葡萄插入缺失标记开发及其在葡萄酒真实性鉴定中的初步验证

孙倩,李娜,杨冰艺,王杰,王晓楠,薄文浩,邢冉冉,程金新,朱保庆   

  1. (1.中国人民警察大学侦查学院,河北?廊坊 065000;2.北京林业大学森林食品与健康研究院,林业食品加工与安全北京市重点实验室,北京 100083;3.中国检验检疫科学研究院,北京 100176)
  • 出版日期:2025-09-25 发布日期:2025-08-19
  • 基金资助:
    中国人民警察大学校级重点专项(ZDZX202101)

Development of Insertion-Deletion Markers for Cabernet Sauvignon Grapes Based on Whole Genome Resequencing and Their Usefulness in Wine Authentication

SUN Qian, LI Na, YANG Bingyi, WANG Jie, WANG Xiaonan, BO Wenhao, XING Ranran, CHENG Jinxin, ZHU Baoqing   

  1. (1. Academy of Investigation, China People’s Police University, Langfang 065000, China; 2. Forest Food and Health Research Institute, Beijing Key Laboratory of Forest Food Processing and Safety, Beijing Forestry University, Beijing 100083, China; 3. Chinese Academy of Inspection and Quarantine, Beijing 100176, China)
  • Online:2025-09-25 Published:2025-08-19

摘要: 本研究通过基因组重测序筛选可实现赤霞珠品种鉴定的插入缺失(insertion-deletion,InDel)标记,并进一步验证其在赤霞珠葡萄酒真实性鉴定中的应用效果。结果表明,在全基因组范围筛选的13 个标记中,经聚合酶链式反应验证后,成功开发出9 个具有多态性且扩增效果良好的InDel标记(Del-1~6、In-1、In-4、In-5),其中有7 个InDel标记(Del-2~6、In-1、In-5)能够区分赤霞珠和马瑟兰品种。通过研究这些InDel标记在葡萄酒中的应用效果,发现3 个InDel标记(Del-2、Del-4、Del-6)能用于鉴别赤霞珠和马瑟兰两款葡萄酒的真实性。本研究结果丰富了葡萄的分子标记,可为InDel标记在葡萄资源遗传多样性、品种鉴定、指纹图谱构建等遗传研究中的利用提供理论基础。

关键词: 插入缺失分子标记;赤霞珠葡萄;品种鉴定;赤霞珠葡萄酒;真实性鉴定

Abstract: This study used genomic resequencing to screen for insertion-deletion (InDel) markers that enable the identification of Cabernet Sauvignon grapes and examined their application effectiveness in Cabernet Sauvignon wine authentication. The results showed that among 13 markers selected from the whole genome, 9 InDel markers (Del-1–6, In-1, and In-4–5) with polymorphism and good amplification effect were successfully developed and validated by polymerase chain reaction (PCR). Del-2–6, In-1, and In-5 were able to distinguish between Cabernet Sauvignon and Marceline grapes. It was found that Del-2, Del-4, Del-6 could be used to distinguish between Cabernet Sauvignon and Marceline wines. These results enrich the molecular markers of grapes, providing a theoretical basis for the application of InDel markers in genetic research such as grape resource genetic diversity, variety identification, and DNA fingerprinting.

Key words: insertion-deletion molecular marker; Cabernet Sauvignon grapes; variety identification; Cabernet Sauvignon wine; authentication

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