梳唇石斛茎和花的体外抗氧化、降血糖活性及代谢组学分析

doi:10.7506/spkx1002-6630-20250318-139

食品科学 ›› 2025, Vol. 46 ›› Issue (21): 18-29.doi: 10.7506/spkx1002-6630-20250318-139

• 典型药食同源功能食品及其健康效应专栏 • 上一篇

梳唇石斛茎和花的体外抗氧化、降血糖活性及代谢组学分析

许馨宁，沙荣双，张雪春，唐军荣，周莹，李能波，何霞红，王振兴

（1.西南林业大学生物与食品工程学院，云南省林下资源保护与利用重点实验室，云南省高校大健康类森林资源开发利用工程研究中心，云南?昆明 650224；2.龙陵县石斛研究所，云南?保山 678300）

发布日期:2025-11-10
基金资助:
现代农业产业技术体系建设专项（CARS-21）；云南省“兴滇英才支持计划”青年人才项目（XDYC-QNRC-2022-0166；XDYC-QNRC-2023-0221）；云南省扶雄专家工作站项目（202405AF140094）；龙陵县农民院士科技服务站资助项目（云科协联[2023]9号）

In Vitro Antioxidant and Hypoglycemic Activities and Metabolomics Analysis of Stems and Flowers of Dendrobium strongylanthum Rchb. f.

XU Xinning, SHA Rongshuang, ZHANG Xuechun, TANG Junrong, ZHOU Ying, LI Nengbo, HE Xiahong, WANG Zhenxing

(1. Yunnan Provincial Key Laboratory for Conservation and Utilization of In-forest Resource, Forest Resources Exploitation and Utilization Engineering Research Center for Grand Health of Yunnan Provincial Universities, College of Biological Science and Food Engineering, Southwest Forestry University, Kunming 650224, China;2. Institute of Caulis Dendrobii of Longling County, Baoshan 678300, China)

Published:2025-11-10

摘要/Abstract

摘要： 选取梳唇石斛的茎和花作为研究对象，测定其总酚、总黄酮和多糖含量，以2,2’-联氮-双-3-乙基苯并噻唑啉-6-磺酸（2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)，ABTS）阳离子自由基、1,1-二苯基-2-三硝基苯肼（1,1-diphenyl-2-picrylhydrazyl，DPPH）自由基、羟自由基清除能力和铁离子还原能力等指标，考察其体外抗氧化活性，以α-葡萄糖苷酶和α-淀粉酶抑制能力为指标，评估其体外降血糖活性，并进一步采用超高效液相色谱-质谱技术对其进行非靶向代谢分析。研究结果显示，梳唇石斛花的总酚和总黄酮含量分别为66.56 mg/g和49.12 mg/g，均高于茎（P＜0.05）；而茎中的总多糖含量为172.78 mg/g，显著高于花（P＜0.05）；抗氧化活性测试表明，花清除DPPH自由基和羟自由基的半抑制浓度分别为2.87 mg/mL和18.42 mg/mL，ABTS阳离子自由基清除能力和铁离子还原能力分别为0.63 mg/g和6.11 mg/g，均优于茎。在降血糖活性方面，花对α-葡萄糖苷酶和α-淀粉酶的抑制能力显著优于茎，分别为28.63 mg/g和35.54 mg/g。通过非靶向代谢组学分析，从梳唇石斛茎和花中共鉴定出1 800 种代谢物，其中589 种为差异代谢物，主要包括羧酸及其衍生物、杂环化合物和氧/硫/氮/氟/磷衍生物等代谢物。关联分析表明，芦丁等类黄酮成分可能是决定梳唇石斛抗氧化和降血糖活性的主要成分。综上所述，梳唇石斛尤其是其花展现出优良的缓解氧化应激和降血糖潜力，可开发为功能性食品原料及天然抗氧化剂。

关键词: 梳唇石斛；花；茎；抗氧化；降血糖；代谢组学

Abstract: In this study, the stems and flowers of Dendrobium strongylanthum Rchb. f. were evaluated for total contents of phenols, total flavonoids, and polysaccharides. The in vitro antioxidant activity was assessed by measuring their 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) cation radical, 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical scavenging capacity, and iron reducing ability; the in vitro hypoglycemic activity was assessed using the α-glucosidase and α-amylase inhibitory assays. Furthermore, untargeted metabolomic analysis was performed using ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS). The results showed that the contents of total phenols and total flavonoids in the flowers (66.56 and 49.12 mg/g, respectively) were higher than those of the stems (P < 0.05), while the polysaccharide content of the stems (172.78 mg/g) was higher than that of the flowers (P < 0.05). The flower extract had stronger antioxidant activity than did the stem extract with half-maximal inhibitory concentration (IC50) of 2.87 and 18.42 mg/mL for DPPH and hydroxyl radicals, respectively and ABTS cation radical scavenging capacity and iron reducing ability of 0.63 mg/g and 6.11 mg/g, respectively. The α-glucosidase and α-amylase inhibitory activity (28.63 and 35.54 mg/g) of the flower extract was higher than that of the stem extract. A total of 1 800 metabolites were identified from the stems and flowers by non-targeted metabolomics analysis, of which 589 were recognized as differential metabolites, includes carboxylic acids and their derivatives, heterocyclic compounds, and oxygen/sulfur/nitrogen/fluorine/phosphorus derivatives. Correlation analysis showed that flavonoid components such as rutin were the major contributors to the antioxidant and hypoglycemic activity of D. strongylanthum Rchb. f.. In conclusion, this plant’s flowers exhibited excellent antioxidant and hypoglycemic activities, indicating its potential as a functional food ingredient and natural antioxidant.

Key words: Dendrobium strongylanthum Rchb. f.; flowers; stems; antioxidant; hypoglycemic; metabolomics

中图分类号:

TS201.4

许馨宁，沙荣双，张雪春，唐军荣，周莹，李能波，何霞红，王振兴. 梳唇石斛茎和花的体外抗氧化、降血糖活性及代谢组学分析[J]. 食品科学, 2025, 46(21): 18-29.

XU Xinning, SHA Rongshuang, ZHANG Xuechun, TANG Junrong, ZHOU Ying, LI Nengbo, HE Xiahong, WANG Zhenxing. In Vitro Antioxidant and Hypoglycemic Activities and Metabolomics Analysis of Stems and Flowers of Dendrobium strongylanthum Rchb. f.[J]. FOOD SCIENCE, 2025, 46(21): 18-29.

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梳唇石斛茎和花的体外抗氧化、降血糖活性及代谢组学分析

In Vitro Antioxidant and Hypoglycemic Activities and Metabolomics Analysis of Stems and Flowers of Dendrobium strongylanthum Rchb. f.

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