食品科学 ›› 2026, Vol. 47 ›› Issue (7): 243-250.doi: 10.7506/spkx1002-6630-20251014-076

• 食品工程 • 上一篇    下一篇

基于高温流化构建绿豆淀粉的蛋白屏障及其对淀粉特性的影响

李永富,陈棋稳,黄金荣,袁岩,史锋   

  1. (1.江南大学食品学院,江苏 无锡 214122;2.可口好粮(无锡)科技有限公司,江苏 无锡 214177;3.江南大学生物工程学院,江苏 无锡 214122)
  • 出版日期:2026-04-15 发布日期:2026-05-08
  • 基金资助:
    “十四五”国家重点研发计划重点专项(2023YFD2100803)

Construction of Protein Barrier around Mung Bean Starch Based on High-temperature Fluidization and Its Effect on Starch Properties

LI Yongfu, CHEN Qiwen, HUANG Jinrong, YUAN Yan, SHI Feng   

  1. (1. School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; 2. NutriGrain (Wuxi) Technology Co. Ltd., Wuxi 214177, China; 3. School of Biotechnology, Jiangnan University, Wuxi 214122, China)
  • Online:2026-04-15 Published:2026-05-08

摘要: 将绿豆在160、180、200 ℃条件下进行瞬时高温流化处理,通过解析处理前后籽粒微观结构、蛋白质、淀粉性质探究其对淀粉消化性的影响。结果表明,处理后绿豆淀粉颗粒形态基本保持完整;淀粉表面出现絮状物且体积随着流化温度升高而增加;绿豆中蛋白质网络逐渐收缩并胁迫淀粉颗粒聚集成团,生成紧密包裹淀粉团的蛋白质屏障层。高温流化对蛋白质二级结构没有显著影响(P<0.05),但二硫键含量随温度的升高先降后升;处理前后绿豆总淀粉含量无显著变化,但直链淀粉质量分数由30.09%增加到最高40.17%,淀粉晶型均为A型,相对结晶度由22.38%降低到15.86%,短程有序性也显著降低。同时,处理后淀粉糊化特征温度、糊化焓以及黏度特性参数均显著下降,蒸煮性能明显改善;绿豆处理后抗性淀粉含量最多增加了13.52%,预估血糖生成指数从51.75降低到44.54。综上,高温流化是一种温和的处理方式,且能生成减慢淀粉消化的蛋白质屏障层,显著增加直链淀粉含量,从而实现对淀粉消化性的有效调控,这为绿豆功能食品开发提供了技术支撑。

关键词: 绿豆;高温流化;淀粉的蛋白质屏障;淀粉结构;淀粉消化性

Abstract: In this study, mung bean was subjected to instant high-temperature fluidization treatment at 160, 180, or 200 ℃. The effects on the starch digestibility of mung bean were investigated by analyzing alterations in microstructure, protein, and starch properties before and after the treatment. The results showed that the starch granules remained intact after the treatment. Flocs appeared on the starch surface and their volume rose as the temperature increased. The protein network in mung bean gradually contracted, causing starch granules to aggregate into clusters and forming a compact protein barrier layer encapsulating the starch aggregates. High-temperature fluidization had no significant effect on the secondary structure of proteins (P < 0.05), while the disulfide bond content initially decreased and then increased with increasing temperature. The total starch content and crystal type (A-type) of mung bean remained unchanged after the treatment. However, the amylose content increased from 30.09% to 40.17%, the crystallinity decreased from 22.38% to 15.86%, and the short-range order of starch significantly reduced. Meanwhile, the gelatinization temperature, gelatinization enthalpy, and pasting parameters decreased significantly, indicating markedly improved cooking properties. The resistant starch content increased by 13.52%, and the estimated glycemic index decreased from 51.75 to 44.54. In conclusion, high-temperature fluidization is a mild processing method that can generate a protein barrier layer around starch to slow down starch digestion and significantly increase amylose content, thereby achieving effective regulation of starch digestibility. This study provides technical support for the development of functional foods based on mung bean.

Key words: mung bean; high-temperature fluidization; protein barrier around starch; starch structure; starch digestibility

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