食品科学 ›› 2026, Vol. 47 ›› Issue (13): 143-152.doi: 10.7506/spkx1002-6630-20251208-076

• 生物工程 • 上一篇    下一篇

川味冷风干香肠风干过程中微生物群落结构及风味物质变化

王欢,钱鑫,陈海,张宇昊   

  1. (1.西南大学食品科学学院,重庆 400715;2.重庆市钱江食品(集团)有限公司,重庆 400000;3.川渝共建特色食品重庆市重点实验室,重庆 400715;4.重庆市农业科学院,重庆 401329)
  • 出版日期:2026-07-15 发布日期:2026-07-17
  • 基金资助:
    国家自然科学基金联合基金项目(U24A20472);国家自然科学基金面上项目(32472254); 重庆市技术创新与应用发展专项重点项目(CSTB2023TIAD-KPX0036)

Changes in Microbial Community Structure and Flavor Compounds during Cold Air-Drying of Sichuan-Style Sausage

WANG Huan, QIAN Xin, CHEN Hai, ZHANG Yuhao   

  1. (1. College of Food Science, Southwest University, Chongqing 400715, China; 2. Chongqing Qianjiang Food Co. Ltd., Chongqing 400000, China; 3. Chongqing Key Laboratory of Speciality Food Co-built by Sichuan and Chongqing, Chongqing 400715, China; 4. Chongqing Academy of Agricultural Sciences, Chongqing 401329, China)
  • Online:2026-07-15 Published:2026-07-17

摘要: 为探究川味冷风干香肠风干过程中微生物菌群结构、风味物质的变化规律及两者的相关性,采用高通量测序技术测定不同风干时间(0~6 d)的冷风干香肠菌群结构,并采用气相色谱-质谱联用技术测定其挥发性风味物质,最后通过相关性分析探明微生物群落结构变化与风味物质形成的内在关联。结果表明,川味冷风干香肠细菌群落的物种丰富度及细菌群落组成在风干过程中变化显著,变形菌门和厚壁菌门是加工过程中的优势菌门,其中有益菌葡萄球菌的相对丰度随着风干时间延长先升高后降低。风干过程中,冷风干香肠的挥发性风味物质发生了显著变化,相对气味活度值分析结果表明,冷风干香肠中有19 种关键风味化合物,包含6 种烯烃类、3 种醇类、4 种酯类、6 种醛类化合物。相关性分析结果表明,葡萄球菌与部分醇类、酯类物质含量呈显著正相关,因此葡萄球菌与冷风干香肠中风味物质的生成密切相关。

关键词: 川味冷风干香肠;风干过程;微生物群落;风味物质;相关性

Abstract: To investigate the changes in microbial community structure and flavor compounds during the cold air-drying of Sichuan-style sausage and to explore the correlation between them, this study employed high-throughput sequencing technology to analyze the bacterial community structure at different drying times (0–6 d). Meanwhile, gas chromatography-mass spectrometry was employed to identify the volatile flavor compounds. Finally, Pearson correlation analysis was conducted to reveal the intrinsic relationship between microbial community dynamics and flavor formation. Results indicated that the species richness and composition of the bacterial community in Sichuan-style sausage significantly varied during the drying process. Proteobacteria and Firmicutes were the dominant phyla, with the relative abundance of the beneficial bacterium Staphylococcus initially increasing and subsequently decreasing as the air-drying time increased. The composition of volatile flavor compounds changed significantly during the air-drying process. Based on relative odor activity value (ROAV), 19 key volatile flavor compounds were identified including six olefinic compounds, three alcohols, four esters, and six aldehydes. The correlation analysis indicated a significant positive correlation between Staphylococcus and the contents of certain alcohols and esters, indicating close association of Staphylococcus with the formation of flavor compounds in cold air-dried sausage.

Key words: Sichuan-style cold air-dried sausage; air-drying process; microbial community; flavor compounds; correlation

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