食品科学 ›› 2026, Vol. 47 ›› Issue (16): 81-93.doi: 10.7506/spkx1002-6630-20260209-073

• 生物工程 • 上一篇    下一篇

食盐添加量对发酵酸鱼微生物群落和风味品质影响及相关性分析

李珂,曾佳坤,闫子康,李宗军   

  1. (湖南农业大学食品科学技术学院,湖南?长沙 410128)
  • 出版日期:2026-08-25 发布日期:2026-09-03
  • 基金资助:
    湖南省重点研发计划项目(2022NK2040)

Effect of Salt Addition on Microbial Community and Flavor Quality in Fermented Sour Fish and Their Correlation Analysis

LI Ke, ZENG Jiakun, YAN Zikang, LI Zongjun   

  1. (School of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China)
  • Online:2026-08-25 Published:2026-09-03

摘要: 本研究设置3%(K3)、6%(K6)、9%(K9)3 种食盐添加量,结合高通量测序、气相色谱-质谱联用等技术,系统探究食盐添加量对酸鱼发酵过程中微生物群落动态变化、风味物质积累及二者相关性的影响。结果表明,食盐添加量显著调控微生物群落结构与多样性,厚壁菌门为发酵过程中的优势菌门,不同盐浓度组核心优势菌属存在差异;低盐组(K3)酸类物质含量更高,而中高盐组(K6、K9)挥发性风味物质总量显著高于低盐组;共鉴定出93 种挥发性风味成分,酯类、醇类、醛类为主要贡献者,不同盐浓度组特征风味化合物存在特异性;微生物与特征风味物质呈现显著特异性相关性。本研究为酸鱼生产工艺标准化及风味优化提供理论依据与实践指导。

关键词: 发酵酸鱼;食盐添加量;微生物群落;风味品质;相关性分析

Abstract: In this study, the effects of salt concentration (3% (K3), 6% (K6), and 9% (K9)) on the dynamic changes in microbial communities, the accumulation of flavor compounds, and the correlation between them during sour fish fermentation were investigated using high-throughput sequencing and gas chromatography-mass spectrometry (GC-MS). The results showed that salt addition significantly regulated the structure and diversity of the microbial community. Firmicutes was the dominant phylum during fermentation, while the core dominant genera varied with salt concentration. The low-salt group (K3) exhibited higher contents of organic acids, whereas ​the total content of volatile flavor substances was significantly higher in the medium- and high-salt groups (K6 and K9) than in the low-salt group. A total of 93 volatile flavor compounds were identified, with esters, alcohols, and aldehydes being the major contributors. Characteristic flavor compounds showed salt concentration specificity. Moreover, a significant and specific correlation was observed between microorganisms and characteristic flavor substances. This study provides a theoretical basis and practical guidance for the standardization of sour fish production and the optimization of its flavor.

Key words: fermented sour fish; salt addition; microbial community; flavor quality; correlation analysis

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