食品科学 ›› 2010, Vol. 31 ›› Issue (23): 61-64.doi: 10.7506/spkx1002-6630-201023015

• 基础研究 • 上一篇    下一篇

富硒蛹虫草抗氧化作用研究

李华为1,赵素云2,铁 梅2,*   

  1. 1.沈阳师范大学化学与生命科学学院
    2.辽宁大学环境学院
  • 收稿日期:2010-09-21 出版日期:2010-12-15 发布日期:2010-12-29
  • 通讯作者: 铁梅 E-mail:tiemei07@yahoo.cn
  • 基金资助:

    国家自然科学基金项目(30671176);辽宁省教育厅科学技术项目(2008227)

Antioxidant Activity of Selenium-rich Cordyceps militaris

LI Hua-wei1,ZHAO Su-yun2,TIE Mei2,*   

  1. 1. College of Chemistry and Life Sciences, Shenyang Normal University, Shenyang 110034, China;
    2. School of Environmental Science, Liaoning University, Shenyang 110036, China
  • Received:2010-09-21 Online:2010-12-15 Published:2010-12-29
  • Contact: TIE Mei2,* E-mail:tiemei07@yahoo.cn

摘要:

从富硒蛹虫草中提取硒多糖和硒蛋白。分别采用ICP-MS 法和苯酚硫酸法及考马斯亮蓝法对硒多糖和硒蛋白中硒、多糖及蛋白含量进行测定;在Fenton 体系中,以510nm 波长处的吸光度研究硒多糖和硒蛋白对羟自由基的清除作用。结果表明:硒多糖和硒蛋白清除羟自由基的效果强于相同浓度的无机硒、多糖及蛋白;其中,富硒蛹虫草硒多糖的对羟自由基最高清除率为38.02%,而硒蛋白的最高清除率可达75.00%。

关键词: 蛹虫草, 硒多糖, 硒蛋白, Fenton 体系, 羟自由基

Abstract:

In this study, selenium polysaccharide and selenium protein were extracted from selenium-rich Cordyceps militaris by modern separation techniques. The contents of selenium, polysaccharide and protein in Se-polysaccharide and Se-protein were determined by ICP-MS, phenol-sulfuric acid method and Coomassie brilliant blue method. In addition, the scavenging capabilities of Se-polysaccharide and Se-protein on hydroxyl free radicals were evaluated by the absorbance at 510 nm in Fenton system. Results indicated that the scavenging activities of Se-polysaccharide and Se-protein were obvious higher than those of inorganic selenium, polysaccharide and protein under the same condition. The highest scavenging rates of Se-polysaccharide and Seprotein were 38.02% and 75.00%, respectively.

Key words: Cordyceps militaris, Se-polysaccharide, Se-protein, Fenton system, hydroxyl free radical

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