蛹虫草胞外多糖的制备、结构分析及其免疫活性

doi:10.7506/spkx1002-6630-20201017-156

食品科学 ›› 2021, Vol. 42 ›› Issue (23): 106-113.doi: 10.7506/spkx1002-6630-20201017-156

蛹虫草胞外多糖的制备、结构分析及其免疫活性

于悦，陈卓，王亚非，张明泽，王亚慧，艾楠，沈明浩，姜斌

（1.吉林农业大学食品科学与工程学院，吉林?长春 130118；2.讷河市人民医院，黑龙江?讷河 161300）

出版日期:2021-12-15 发布日期:2021-12-29
基金资助:
吉林省教育厅科学技术重点项目（JKH20210342KJ）

Preparation, Structure and Immuneomodulatory Activity of Exopolysaccharide from Cordyceps militaris

YU Yue, CHEN Zhuo, WANG Yafei, ZHANG Mingze, WANG Yahui, AI Nan, SHEN Minghao, JIANG Bin

(1. College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China; 2. Nehe People’s Hospital, Nehe 161300, China)

Online:2021-12-15 Published:2021-12-29

摘要/Abstract

摘要： 本实验选择蛹虫草菌株CICC 14014液态发酵培养，提取蛹虫草胞外多糖，测定其理化性质及结构，并对其免疫活性进行研究。采用DEAE-Sephacel阴离子交换柱联合Sephadex G-200葡聚糖凝胶柱纯化蛹虫草胞外多糖；利用高效液相色谱法测定多糖分子质量，利用傅里叶变换红外光谱鉴定多糖结构；选用BALB/c小鼠构建免疫损伤模型，考察其体质量及免疫器官指数变化情况，观察其脾脏组织切片形态，计算其T、B细胞增殖率，测定其血清细胞因子及免疫球蛋白质量浓度。结果表明，蛹虫草发酵液中多糖质量浓度为3.15 mg/mL，经分离纯化后所得单一多糖分子质量为3.67 kDa，纯度可达86.13%。动物实验结果表明，蛹虫草胞外多糖可明显提高免疫器官指数，能够有效促使T细胞（低剂量时）、B细胞增殖和免疫球蛋白（immunoglobulin，Ig）G、IgA、IgM、肿瘤坏死因子-α、白细胞介素（interleukin，IL）-6、IL-2、干扰素（interferon，IFN）-γ的分泌，免疫调节效果显著。研究结果表明蛹虫草胞外多糖可修复环磷酰胺导致的BALB/c小鼠免疫功能损伤，为开发蛹虫草发酵液资源建立了部分理论支撑。

关键词: 蛹虫草胞外多糖；多糖结构；环磷酰胺；脾淋巴细胞增殖；免疫调节活性

Abstract: An exopolysaccharide from the fermentation supernatant of Cordyceps militaris strain 14014 from China Center of Industrial Culture Collection (CICC) was extracted and purified by sequential column chromatography on DEAE-Sephacel and Sephadex G-200. The molecular mass of the polysaccharide was determined by high performance liquid chromatography (HPLC) and it was structurally identified by Fourier transform infrared (FTIR) spectroscopy. Meanwhile, the immunostimulatory activity?of the polysaccharide in immunosuppressed BALB/c mice was evaluated by measuring the changes in body mass and immune organ indexes, observing spleen tissue sections, calculating the proliferation rate of spleen lymphocytes, and determining the contents of serum cytokines and immunoglobulins (Ig). The results showed that the polysaccharide content in the fermentation broth of Cordyceps militaris was 3.15 mg/mL, the molecular mass of the purified polysaccharide was 3.67 kDa, and its purity was 86.13%. The exopolysaccharide from Cordyceps militaris significantly improved immune organ indexes, promoted the proliferation of T (at low dose) and B cells, and stimulated the secretion of tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-2, interferon (IFN)-γ, IgG, IgA and IgM in mice, indicating a good immunoregulatory effect. The above results indicated that the exoposaccharide could repair cyclophosphamide-induced damage to immune functions in BALB/c mice. This finding may provide a theoretical basis for the development and utilization of Cordyceps militaris fermentation broth.

Key words: exopolysaccharide from Cordyceps militaris; polysaccharide structure; cyclophosphamide; spleen lymphocyte proliferation; immunoregulatory activity

中图分类号:

TS201.4

于悦，陈卓，王亚非，张明泽，王亚慧，艾楠，沈明浩，姜斌. 蛹虫草胞外多糖的制备、结构分析及其免疫活性[J]. 食品科学, 2021, 42(23): 106-113.

YU Yue, CHEN Zhuo, WANG Yafei, ZHANG Mingze, WANG Yahui, AI Nan, SHEN Minghao, JIANG Bin. Preparation, Structure and Immuneomodulatory Activity of Exopolysaccharide from Cordyceps militaris[J]. FOOD SCIENCE, 2021, 42(23): 106-113.

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蛹虫草胞外多糖的制备、结构分析及其免疫活性

Preparation, Structure and Immuneomodulatory Activity of Exopolysaccharide from Cordyceps militaris

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