EGCG通过激活AMPK、抑制PPARγ调控3T3-L1脂肪细胞中脂滴蓄积

doi:10.7506/spkx1002-6630-20201221-234

食品科学 ›› 2021, Vol. 42 ›› Issue (23): 152-161.doi: 10.7506/spkx1002-6630-20201221-234

EGCG通过激活AMPK、抑制PPARγ调控3T3-L1脂肪细胞中脂滴蓄积

管巧丽，吴晓云，李芳，盛军

（1.云南农业大学普洱茶学教育部重点实验室，云南?昆明 650201；2.云南农业大学食品科学技术学院，云南?昆明 650201；3.云南农业大学理学院，云南?昆明 650201）

出版日期:2021-12-15 发布日期:2021-12-29
基金资助:
国家自然科学基金地区科学基金项目（81760667）；云南省重大科技专项计划项目（2018ZG013）；云南省重大科技专项计划项目（2018ZG010）

Epigallocatechin Gallate (EGCG) Regulates Lipid Droplet Accumulation in 3T3-L1 Adipocytes through Activating Adenosine Monophosphate-Activated Protein Kinase (AMPK) and Consequently Inhibiting Peroxisome Proliferator Activated Receptor-γ (PPARγ)

GUAN Qiaoli, WU Xiaoyun, LI Fang, SHENG Jun

(1. Key Laboratory of Pu-er Tea Science, Ministry of Education, Yunnan Agricultural University, Kunming 650201, China; 2. College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China; 3. College of Science, Yunnan Agricultural University, Kunming 650201, China)

Online:2021-12-15 Published:2021-12-29

摘要/Abstract

摘要： 研究表没食子儿茶素没食子酸酯（epigallocatechin gallate，EGCG）对3T3-L1成熟脂肪细胞脂滴蓄积的影响。通过体外诱导分化培养3T3-L1前脂肪细胞，经EGCG处理后，脂肪细胞脂滴大小减小，并表现为时间和浓度依赖性；不会引起成熟脂肪细胞的凋亡。与对照组相比，EGCG处理组显著降低与脂代谢、转运和吸收相关基因的mRNA的表达水平；EGCG处理可以显著降低过氧化物酶体增殖物激活受体（peroxisome proliferators-activated receptor-γ，PPARγ）的表达并激活腺苷一磷酸（adenosine monophosphate，AMP)活化蛋白激酶（AMP-activated protein kinase，AMPK）。使用AMPK抑制剂Dorsomorphin处理成熟脂肪细胞，发现与单独抑制剂组相比，EGCG能提高磷酸化-AMPK的表达水平，并抑制PPARγ的表达。结论：EGCG可能通过激活AMPK从而抑制PPARγ的表达并显著降低与脂代谢、转运和吸收相关基因的mRNA的表达水平，从而减少3T3-L1成熟脂肪细胞的脂滴蓄积。

关键词: 表没食子儿茶素没食子酸酯；3T3-L1脂肪细胞；AMP活化蛋白激酶；过氧化物酶体增殖物激活受体；脂滴蓄积

Abstract: In this study, we explored the effect of epigallocatechin gallate (EGCG) on lipid droplet accumulation in mature 3T3-L1 adipocytes. For this purpose, 3T3-L1 preadipocytes were induced to differentiate?into mature adipocytes. After EGCG treatment, the size of lipid droplets formed in the adipocytes decreased in a time and concentration-dependent manner. On the other hand, EGCG didn’t lead to cell apoptosis. Compared with the control group, EGCG treatment significantly down-regulated the mRNA expression levels of the genes associated with lipid metabolism, transport and absorption. EGCG treatment also significantly decreased the expression of peroxisome proliferator-activated receptor-γ (PPARγ) and activated adenosine monophosphate (AMP)-activated protein kinase (AMPK). EGCG up-regulated the expression of phospho-AMPK and inhibited the expression of PPARγ in mature adipocytes further treated with the AMPK inhibitor dorsomorphin. Therefore, EGCG can reduce lipid droplet accumulation in mature 3T3-L1 adipocytes by activating AMPK, consequently inhibiting PPARγ expression, and reducing the mRNA expression of the genes related to lipid metabolism, transport and absorption.

Key words: epigallocatechin gallate; 3T3-L1 adipocytes; AMP-activated protein kinase; peroxisome proliferators-activated receptors; lipid droplet accumulation

中图分类号:

TS201.4

管巧丽，吴晓云，李芳，盛军. EGCG通过激活AMPK、抑制PPARγ调控3T3-L1脂肪细胞中脂滴蓄积[J]. 食品科学, 2021, 42(23): 152-161.

GUAN Qiaoli, WU Xiaoyun, LI Fang, SHENG Jun. Epigallocatechin Gallate (EGCG) Regulates Lipid Droplet Accumulation in 3T3-L1 Adipocytes through Activating Adenosine Monophosphate-Activated Protein Kinase (AMPK) and Consequently Inhibiting Peroxisome Proliferator Activated Receptor-γ (PPARγ)[J]. FOOD SCIENCE, 2021, 42(23): 152-161.

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EGCG通过激活AMPK、抑制PPARγ调控3T3-L1脂肪细胞中脂滴蓄积

Epigallocatechin Gallate (EGCG) Regulates Lipid Droplet Accumulation in 3T3-L1 Adipocytes through Activating Adenosine Monophosphate-Activated Protein Kinase (AMPK) and Consequently Inhibiting Peroxisome Proliferator Activated Receptor-γ (PPARγ)

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