金针菇膳食纤维改性、理化性质及抗氧化、降血脂活性

doi:10.7506/spkx1002-6630-20201020-199

食品科学 ›› 2021, Vol. 42 ›› Issue (23): 90-98.doi: 10.7506/spkx1002-6630-20201020-199

金针菇膳食纤维改性、理化性质及抗氧化、降血脂活性

刘学成，王文亮，弓志青，王延圣，崔文甲，宋莎莎，张剑，贾凤娟

（山东省农业科学院农产品加工与营养研究所，山东省农产品精深加工技术重点实验室，农业农村部新食品资源加工重点实验室，山东?济南 250100）

出版日期:2021-12-15 发布日期:2021-12-29
基金资助:
山东省重大科技创新工程项目（2019JZZY020607）；山东省农业科学院（邹城）食药用菌产业技术研究院产学研合作项目；山东省基金重大基础研究项目（ZR2018ZC0944）；山东省现代农业产业技术体系食用菌产后加工岗位专家项目（SDAIT-07-09）

Modification, Physicochemical Properties and Lipid-Lowering and Antioxidant Activity of Dietary Fiber from Flammulina velutipes

LIU Xuecheng, WANG Wenliang, GONG Zhiqing, WANG Yansheng, CUI Wenjia, SONG Shasha, ZHANG Jian, JIA Fengjuan

(Key Laboratory of Agro-products Deep Processing Technology of Shandong Province, Key Laboratory of Novel Food Resources Processing, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Products Processing and Nutrition, Shandong Academy of Agricultural Sciences, Jinan 250100, China)

Online:2021-12-15 Published:2021-12-29

摘要/Abstract

摘要： 以金针菇膳食纤维（dietary fiber，DF）为原料（其中可溶性膳食纤维（soluble dietary fiber，SDF）质量分数8.2%），分别采用纤维素酶法和高温蒸煮法对其进行改性处理，在单因素试验基础上通过正交优化试验得到改性最佳工艺优化参数。纤维素酶法处理最佳工艺为液料比35∶1、纤维素酶用量1.5%（以体系质量计）、酶解时间2 h，在此工艺下SDF得率为16.2%。高温蒸煮法处理最佳工艺为液料比30∶1、蒸煮温度125 ℃、蒸煮时间50 min，在此工艺下SDF得率为20.4%。将以上两种改性方法得到的改性DF与未改性DF进行理化性质（包括阳离子交换力、胆固醇吸附力和葡萄糖吸收能力）对比，并进行扫描电子显微镜和傅里叶变换红外光谱分析，结果发现高温蒸煮改性DF整体上各项性质均优于纤维素酶改性DF。此外，建立小鼠高脂肥胖模型，利用高温蒸煮改性后的金针菇DF饲料饲喂小鼠，通过对小鼠体质量，血脂中甘油三酯、总胆固醇、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇，血清中超氧化物歧化酶和谷胱甘肽过氧化物酶等指标水平的测定评价改性DF的生理活性，结果显示添加高温蒸煮改性DF能够改善高脂肥胖小鼠的生理指标，说明改性后的金针菇DF保持了良好的生理活性。

关键词: 膳食纤维；改性；理化性质；生理活性

Abstract: Dietary fiber (DF) containing 8.2% soluble dietary fiber (SDF) extracted from Flammulina velutipes was modified by cellulase or high-temperature cooking. As determined using one-factor-at-a-time and orthogonal array designs, the optimal processing parameters for the cellulase method were as follows: liquid-to-material ratio 35:1, enzyme dosage 1.5%, and hydrolysis time 2 h, giving an SDF yield of 16.2%. The optimal processing parameters for the high-temperature cooking method were liquid-to-material ratio 30:1, temperature 125 ℃, and time 50 min, providing an SDF yield of 20.4%. The cation exchange capacity, cholesterol adsorption capacity, and glucose absorption capacity of the modified DFs were compared with those of the unmodified one, and further comparative analyses were performed using scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy. It was found that the autoclaved DF was better than the cellulase-treated DF in general. Furthermore, mice with obesity induced by a high-fat diet were intervened with the autoclaved DF. Body mass, as well as triglycerides (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in serum were measured. It was found that the autoclaved DF could improve the physiological indexes of obese mice, indicating its good bioactivity.

Key words: dietary fiber; modification; physicochemical properties; physiological activity

中图分类号:

TS201.4

刘学成，王文亮，弓志青，王延圣，崔文甲，宋莎莎，张剑，贾凤娟. 金针菇膳食纤维改性、理化性质及抗氧化、降血脂活性[J]. 食品科学, 2021, 42(23): 90-98.

LIU Xuecheng, WANG Wenliang, GONG Zhiqing, WANG Yansheng, CUI Wenjia, SONG Shasha, ZHANG Jian, JIA Fengjuan. Modification, Physicochemical Properties and Lipid-Lowering and Antioxidant Activity of Dietary Fiber from Flammulina velutipes[J]. FOOD SCIENCE, 2021, 42(23): 90-98.

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金针菇膳食纤维改性、理化性质及抗氧化、降血脂活性

Modification, Physicochemical Properties and Lipid-Lowering and Antioxidant Activity of Dietary Fiber from Flammulina velutipes

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