食品科学 ›› 2021, Vol. 42 ›› Issue (23): 170-181.doi: 10.7506/spkx1002-6630-20201021-205

• 营养卫生 • 上一篇    下一篇

‘平阳黄汤’对高脂饮食大鼠肠道屏障和肠道菌群的影响

欧阳建,李秀平,周方,卢丹敏,黄建安,曾呈理,谢前途,钟维标,刘仲华   

  1. (1.国家植物功能成分利用工程技术研究中心,湖南农业大学?茶学教育部重点实验室,植物功能成分利用省部共建协同创新中心,湖南?长沙 410128;2.浙江省平阳县农业农村局,浙江?平阳 325400;3.浙江子久文化股份有限公司,浙江?平阳 325400)
  • 出版日期:2021-12-15 发布日期:2021-12-29
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2017YFD0400803); 财政部和农业农村部:现代农业产业技术体系建设专项(CARS-19-09B)

Effect of Pingyang Yellow Tea on Intestinal Barrier and Intestinal Flora in Rats Fed High-Fat Diet

OUYANG Jian, LI Xiuping, ZHOU Fang, LU Danmin, HUANG Jian’an, ZENG Chengli, XIE Qiantu, ZHONG Weibiao, LIU Zhonghua   

  1. (1. National Research Center of Engineering and Technology for Utilization Ingredients from Botanicals, Key Laboratory for Tea Science, Ministry of Education, Hunan Agricultural University, Co-innovation Center of Education Ministry for Utilization of Botanical Functional Ingredients, Changsha 410128, China; 2. Agriculture and Rural Affairs Bureau of Pingyang County, Pingyang 325400, China; 3. Zhejiang Zijiu Culture Co., Ltd., Pingyang 325400, China)
  • Online:2021-12-15 Published:2021-12-29

摘要: 为探讨‘平阳黄汤’对高脂饮食大鼠的肥胖调控作用及其与肠道屏障和菌群的关系,将SD雄性大鼠分为正常饮食组、高脂饮食组以及平阳黄汤低、中、高剂量组(75、150、300 mg/kg mb),建立预防肥胖模型。通过分析大鼠体质量变化、摄食量、脂肪指数、血清指标、肝脏指标和肝脏、结肠组织切片评估‘平阳黄汤’对高脂饮食大鼠的肥胖调控作用;通过分析肠道的病理形态、杯状细胞数量和紧密连接蛋白的表达评估肠道屏障作用;通过分析微生物多样性和菌群结构评估对肠道菌群的影响。结果:高剂量‘平阳黄汤’能极显著降低高脂饮食大鼠的体质量增长量、附睾和肾周脂肪质量(P<0.01),降低血清血脂、脂肪因子和氧化应激水平(P<0.01),降低肝脏甘油三酯和总胆固醇水平(P<0.01),有效调节肝脏的脂质堆积和炎症损伤。同时,高剂量平阳黄汤还显著改善了高脂饮食大鼠结肠的形态,减少绒毛脱落和炎症浸润现象,显著恢复肠道杯状细胞数量,提高闭锁小带蛋白和闭锁蛋白的表达(P<0.01),有效降低了血清中脂多糖、肿瘤坏死因子α和白细胞介素6水平(P<0.01),有效预防代谢性内毒素和全身慢性炎症,提高了大鼠肠道菌群的丰度和多样性,促进了与肥胖发生相关有益菌的增殖,抑制有害菌的生长。结论:‘平阳黄汤’能通过维护肠道屏障和调节肠道菌群紊乱,有效调控高脂饮食引起的肥胖。

关键词: 平阳黄汤;肥胖;肠道屏障;肠道菌群

Abstract: This study was intended to explore the regulatory effect of Pingyang yellow tea on high-fat diet (HDF)-induced obesity in rats and its relationship with the intestinal barrier and flora. SD male rats were divided into five groups: normal diet (ND), HFD, and low-, medium- and high-dose tea infusion (75, 150 and 300 mg/kg mb). The regulatory effect of Pingyang yellow tea on obesity was evaluated by analyzing the body mass changes, feed intake, visceral fat indexes, serum biochemical indexes, liver lipids and liver and colon tissue sections of rats. The intestinal barrier function was evaluated in terms of intestinal pathological morphology, the number of goblet cells, and the expression of tight junction proteins, and the effect on the diversity and structure of the intestinal flora was evaluated. The results showed that the high dose of Pingyang yellow tea significantly reduced body mass gain, epididymal fat mass perirenal fat mass of HFD rats (P < 0.01), lowered serum lipids, adipocytokines and oxidative stress levels (P < 0.01) as well as the levels of liver triglyceride (TG) and total cholesterol (TC) (P < 0.01), and effectively regulated liver lipid accumulation and inflammatory damage. At the same time, high-dose Pingyang yellow tea also significantly improved colonic morphology in HFD-fed rats, reduced villus shedding and inflammatory cell infiltration, significantly restored the number of intestinal goblet cells, increased zonula occludens-1 (ZO-1) and occludin expression (P < 0.01), significantly decreased the levels of serum lipopolysaccharide (LPS), tumor necrosis factor (TNF)-α and interleukin (IL)-6 (P < 0.01), effectively prevented metabolic endotoxins and systemic chronic inflammations, increased the abundance and diversity of the intestinal flora, promoted the proliferation of beneficial bacteria related to obesity, and inhibited the growth of harmful bacteria. In summary, Pingyang yellow tea can effectively prevent HFD-induced obesity by maintaining the intestinal barrier and regulating the disturbance of the intestinal flora.

Key words: Pingyang yellow tea; obesity; intestinal barrier; intestinal flora

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