食品科学 ›› 2010, Vol. 31 ›› Issue (21): 24-28.doi: 10.7506/spkx1002-6630-201021005

• 基础研究 • 上一篇    下一篇

十六烷基三甲基溴化铵和乳清蛋白结合的研究

刘 伟,李兴民* ,郑海涛,刘 毅   

  1. 中国农业大学食品科学与营养工程学院,教育部- 北京市共建功能乳品重点实验室
  • 收稿日期:2010-04-23 出版日期:2010-11-15 发布日期:2010-12-29
  • 通讯作者: 李兴民 E-mail:lixingmin@cau.edu.cn
  • 基金资助:

    “十一五”国家科技支撑计划项目(2006BAD04A06)

Effects of Physico-chemical Factors on Complexation of Cetyltrimethyl Ammol/Lonium Bromide with Whey Protein

LIU Wei,LI Xing-min*,ZHENG Hai-tao,LIU Yi   

  1. Key Laboratory of Functional Dairy, Co-constructed by Ministry of Education and Beijing Government, College of Food Science and
    Nutritional Engineering, China Agricultural University, Beijing 100083, China
  • Received:2010-04-23 Online:2010-11-15 Published:2010-12-29
  • Contact: LI Xing-min E-mail:lixingmin@cau.edu.cn

摘要:

寻找新型絮凝剂,研究其与蛋白粒子结合的物化因素,实现蛋白分离及对乳清的合理利用。研究不同因素如十六烷基三甲基溴化铵(CTAB)浓度、pH 值、温度、蛋白质量浓度、离子强度对蛋白-CTAB 复合物形成的影响,获得乳清蛋白与CTAB 结合的最佳物化条件。结果表明:CTAB 添加量是影响蛋白-CTAB 结合的最大因素;不同pH 值对CTAB- 蛋白的影响差异不显著;不同pH 值条件下,温度对CTAB- 蛋白结合影响显著;在0~200mmol/L 范围内,离子强度越高,结合的络合物越少。蛋白质量浓度越大,结合的络合物越多,蛋白回收率越大。

关键词: 乳清蛋白, 十六烷基三甲基溴化铵(CTAB), 静电结合, 物化因素

Abstract:

Today, understanding physico-chemical factors affecting the complexation between coagulant and protein granules for protein separation is of great interest to the finding of novel coagulants and the reasonable utilization of whey protein. The effects of different factors such as final cetyltrimethyl ammol/lonium bromide (CTAB) concentration, pH, temperature, whey protein concentration and ionic intensity on the complexation between CTAB and whey protein were dealt with for finding the best complexation conditions. Final CTAB concentration was found to be the most important factor. pH had no significant effect on CTAB/whey protein complexation. At different pH values, the complexation was significantly affected by temperature. In a range of 0 to 200 mmol/L, higher ionic intensity resulted in a decrease of CTAB/whey protein complexation. However, increasing whey protein concentration affected the complexation in a positive way, resulting in an increase of protein recovery.

Key words: whey protein, CTAB, electrostatic interaction, physico-chemical factors

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