食品科学 ›› 2010, Vol. 31 ›› Issue (21): 357-360.doi: 10.7506/spkx1002-6630-201021081

• 营养卫生 • 上一篇    下一篇

覆盆子糖蛋白的抗氧化作用

田 甜,段玉峰* ,牛付阁   

  1. 陕西师范大学食品工程与营养科学学院
  • 收稿日期:2010-03-02 修回日期:2010-10-17 出版日期:2010-11-15 发布日期:2010-12-29
  • 通讯作者: 段玉峰 E-mail:yfduan@snnu.edu.cn

Antioxidant Effect of Raspberry Glycoprotein

TIAN Tian,DUAN Yu-feng*,NIU Fu-ge   

  1. (College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi an 710062, China)
  • Received:2010-03-02 Revised:2010-10-17 Online:2010-11-15 Published:2010-12-29
  • Contact: DUAN Yu-feng E-mail:yfduan@snnu.edu.cn

摘要:

目的:研究覆盆子糖蛋白的体内外抗氧化作用。方法:通过测定覆盆子糖蛋白粗提物的总还原能力及对羟自由基(·OH)、超氧阴离子自由基(O2 - ·)和DPPH 自由基的清除作用,证明覆盆子糖蛋白粗提物的体外抗氧化作用;并通过测定小鼠血清、肝脏、脑组织中过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性的变化,研究覆盆子糖蛋白粗提物对小鼠体内抗氧化性的影响。结果表明:覆盆子糖蛋白粗提物可显著增强小鼠血清、肝脏、脑组织中CAT、SOD、GSH-Px 活性;并有一定的还原能力,并可有效地清除·OH、O2 - ·和DPPH 自由基。结论:覆盆子糖蛋白具有明显的抗氧化作用。

关键词: 覆盆子, 糖蛋白, 自由基, 抗氧化

Abstract:

Objective: To investigate the antioxidant activity of raspberry glycoprotein (RGP) in vitro and in vivo. Methods: The antioxidant activity of raspberry glycoprotein in vitro was evaluated by determining its reducing power and scavenging capacities against hydroxyl, superoxide anion and DPPH free radicals. The activities of CAT, SOD and GSH-PX in serum, liver and brain tissues of mice were determined to evaluate the antioxidant activity of raspberry glycoprotein in vivo. Results: Raspberry glycoprotein significantly increased the activities of CAT, SOD and GSH-PX in serum, liver and brain tissues of mice, revealed strong reducing power, and effectively eliminated hydroxyl, superoxide anion and DPPH free radicals. Conclusion: Raspberry glycoprotein has obvious antioxidant effect.

Key words: raspberry, glycoprotein, free radical, antioxidation

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