食品科学 ›› 2010, Vol. 31 ›› Issue (19): 45-48.doi: 10.7506/spkx1002-6630-201019007

• 基础研究 • 上一篇    下一篇

苦菜不同部位提取物的抗氧化活性

韩阳阳1,王天晓2,朱海芳1,王 玮1,王建华1 ,*   

  1. 1.山东农业大学生命科学学院
    2.厦门大学材料学院
  • 收稿日期:2009-12-22 出版日期:2010-10-15 发布日期:2010-12-29
  • 通讯作者: 王建华 E-mail:jhwangjh@163.com
  • 基金资助:

    泰安市2007 中药现代化科技专项(5012100999)

Antioxidant Activity Assessment of Extracts from Different Parts of Sonchus oleraceus L.

HAN Yang-yang1,WANG Tian-xiao2,ZHU Hai-fang1,WANG Wei1,WANG Jian-hua1,*   

  1. 1. College of Life Science, Shandong Agricultural University, Tai an 271017, China;
    2. College of Materials, Xiamen University, Xiamen 361005, China
  • Received:2009-12-22 Online:2010-10-15 Published:2010-12-29
  • Contact: WANG Jian-hua1,* E-mail:jhwangjh@163.com

摘要:

分别用水、乙醇(体积分数分别为25%、50%、75%)和无水乙醇,对苦菜的根、茎、叶、花4 个部位进行冷浸提取。测定不同处理条件下的提取率,并用DPPH 自由基清除法检测提取物的抗氧化活性;测定提取物的总黄酮和总多酚类物质的含量及其EC50。结果表明:用50% 乙醇提取的苦菜各部位提取率最高,不同部位不同溶剂提取物的抗氧化活性均呈明显的剂量关系;提取物的抗氧化活性与其中总黄酮和总多酚的含量有关;不同部位的抗氧化能力大小为:花>叶>茎>根。

关键词: 苦菜, 总黄酮, 总多酚, 抗氧化活性

Abstract:

Four different parts of Sonchus oleraceus L. (root, stem, leaf and flower) were extracted separately with water and different concentrations of ethanol (25%, 50%, 75% and 100%) under room temperature. The calculation of extraction efficiency was performed, and the resultant extracts were tested for their DPPH radical scavenging capacity (EC50, half maximal effective concentration) and the contents of total flavonoids. Fifty percent ethanol gave the highest extraction efficiency of ethanol soluble substances from each of these four parts of Sonchus oleraceus L. Different extracts from different parts of Sonchus oleraceus L. all had significantly dosage-dependent antioxidant activity, which was related to the contents of total flavonoids and total polyphenols. The order of antioxidant activity for these four parts was: flower > leaf > stem > root.

Key words: Sonchus oleraceus L., total flavonoids, total polyphenols, antioxidant activity

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