食品科学 ›› 2010, Vol. 31 ›› Issue (18): 50-54.doi: 10.7506/spkx1002-6630-201018012

• 工艺技术 • 上一篇    下一篇

野马追类黄酮的超声提取及其抗氧化活性研究

王卫东,王乃馨,李姣姣,张同天,李 超*   

  1. 徐州工程学院食品工程学院
  • 收稿日期:2009-12-22 出版日期:2010-09-25 发布日期:2010-12-29
  • 通讯作者: 李 超 E-mail:wwd.123@163.com

Ultrasonic Extraction and Antioxidant Activity Evaluation of Flavonoids from the Whole Plant of Eupatorium lindleyanum DC

WANG Wei-dong,WANG Nai-xin,LI Jiao-jiao,ZHANG Tong-tian,LI Chao   

  1. College of Food Engineering, Xuzhou Institute of Technology, Xuzhou 221008, China
  • Received:2009-12-22 Online:2010-09-25 Published:2010-12-29
  • Contact: LI Chao E-mail:wwd.123@163.com

摘要:

在单因素试验的基础上,采用Box-Behnken 试验设计优化超声提取野马追类黄酮的最佳工艺参数,并研究野马追类黄酮对DPPH 自由基的清除效果,最后与其他提取方法进行比较。结果表明:超声提取野马追类黄酮的最佳工艺参数为液料比11.3:1(mL/g)、超声温度58℃和超声时间29min,此时得率为1.142%;当野马追类黄酮质量浓度在6.556~32.78μg/mL 范围内,其对DPPH 自由基的清除率为46.90%~61.06%,且存在明显的量效关系。与其他提取方法比较,超声提取时间短、得率高。

关键词: 野马追, 类黄酮, 超声提取, Box-Behnken 试验设计, 抗氧化活性

Abstract:

Ultrasonic extraction (UE) of flavonoids from the whole plant of Eupatorium lindleyanum DC was optimized using Box-Behnken design and response surface methodology on the basis of single factor experimental results. The scavenging effect of the extract against DPPH free radicals was also studied. Results indicated that the optimal extraction conditions of flavonoids from the whole plant of Eupatorium lindleyanum DC were liquid-to-ratio of 11.3:1, ultrasonic extraction temperature of 58 ℃ and ultrasonic treatment time of 29 min. The extraction rate of flavonoids under these optimal extraction conditions was 1.142%. It was calculated that the content of flavonoids in Eupatorium lindleyanum DC ranged from 6.556 to 32.78 μg/mL. The scavenging rate of flavonoids from Eupatorium lindleyanum DC against DPPH free radicals was between 46.90% and 61.06% in a dosedependent manner. Compared with other extraction techniques, UE exhibited higher yield and shorter extraction time.

Key words: Eupatorium lindleyanum DC, flavonoid, ultrasonic extraction, Box-Behnken design, antioxidant activity

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