酸浆宿萼中β-隐黄质体外淬灭单线态氧能力评价

doi:10.7506/spkx1002-6630-201017036

食品科学 ›› 2010, Vol. 31 ›› Issue (17): 158-161.doi: 10.7506/spkx1002-6630-201017036

酸浆宿萼中β-隐黄质体外淬灭单线态氧能力评价

蔡靳，赵婷，惠伯棣*，宫平

北京联合大学应用文理学院

收稿日期:2010-06-04 修回日期:2010-08-25 出版日期:2010-09-15 发布日期:2010-12-29
通讯作者: 惠伯棣 E-mail:bodi_hui@sohu.com
基金资助:
“十一五”国家科技支撑计划项目(2008BAI58B06)

in vitro Singlet Oxygen Quenching Ability Assessment of β-Cryptoxanthin from the Calyx of Physalis alkengi L. var. franchetii (Mast.) Makino

CAI Jin，ZHAO Ting，HUI Bo-di*，GONG Ping

College of Applied Arts and Science, Beijing Union University, Beijing 100191, China

Received:2010-06-04 Revised:2010-08-25 Online:2010-09-15 Published:2010-12-29
Contact: Bodi HUI E-mail:bodi_hui@sohu.com

摘要/Abstract

摘要：

目的：从酸浆(Physalis alkengi L. var. franchetii (Mast.) Makino)宿萼中制备β- 隐黄质(β-cryptoxanthin)，应用微弱发光测量方法对β- 隐黄质体外淬灭单线态氧的能力进行评价。方法：用正己烷萃取酸浆宿萼中的β- 隐黄质酯后经皂化获得单体，使用C18-HPLC-PDA 对制备物进行定性和定量。用次氯酸钠- 双氧水反应产生单线态氧，使用微弱发光测量仪检测制备组分淬灭单线态氧的能力。结果：经C18-HPLC-PDA 鉴定，制备物中的类胡萝卜素主要为β- 隐黄质。微弱发光测量仪的数据显示：β- 隐黄质的存在能减少次氯酸钠- 双氧水单线态氧体系中产生的最大光子计数，且存在量效关系。0.049～0.49g/L 的β- 隐黄质可减少50% 的最大光子数。

关键词: 宿萼, β-隐黄质, 单线态氧

Abstract:

Objective: To assess the ability of β-cryptoxanthin from the calyx of Physalisalkengi L. var. franchetii (Mast.) Makino to quench in vitro singlet oxygen. Methods: β-cryptoxanthin eater was extracted from the calyx of Physalisalkengi L. var. franchetii (Mast.) Makino with n-hexane and then saponified to produce β-cryptoxanthin fraction. The fraction was identified and quantified on C18-HPLC-PDA. Based on the reaction between NaClO and H2O2, singlet oxygen was produced. Singlet oxygen quenching ability of the β-cryptoxanthin fraction was determined on ultra-weak luminescience analyzer. Results: By C18-HPLCPDA, the prepared fraction was identified as β-cryptoxanthin. The data from ultra-weak luminescience analyzer suggested that the β-cryptoxanthin fraction was able to reduce the maximum number of photon generated from the reaction solution in positive correlation with its amount. β-Cryptoxanthin with an amount ranging between 0.049 g/L and 0.49 g/L resulted in a 50% reduction of maximum photon number.

Key words: calyx, β-cryptoxanthin, singlet-oxygen

中图分类号:

TS264.4

蔡靳，赵婷，惠伯棣，宫平. 酸浆宿萼中β-隐黄质体外淬灭单线态氧能力评价[J]. 食品科学, 2010, 31(17): 158-161.

CAI Jin，ZHAO Ting，HUI Bo-di，GONG Ping. in vitro Singlet Oxygen Quenching Ability Assessment of β-Cryptoxanthin from the Calyx of Physalis alkengi L. var. franchetii (Mast.) Makino[J]. FOOD SCIENCE, 2010, 31(17): 158-161.

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酸浆宿萼中β-隐黄质体外淬灭单线态氧能力评价

in vitro Singlet Oxygen Quenching Ability Assessment of β-Cryptoxanthin from the Calyx of Physalis alkengi L. var. franchetii (Mast.) Makino

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