食品科学 ›› 2010, Vol. 31 ›› Issue (8): 91-96.doi: 10.7506/spkx1002-6300-201008020

• 工艺技术 • 上一篇    下一篇

乳铁蛋白铬络合物制备方法的比较

吴 扬,胡志和*   

  1. 天津市食品生物技术重点实验室,天津商业大学生物技术与食品科学学院
  • 收稿日期:2009-07-08 修回日期:2009-11-19 出版日期:2010-04-15 发布日期:2010-12-29
  • 通讯作者: 胡志和 E-mail:hzihe@tjcu.edu.cn
  • 基金资助:

    天津市重点科技支撑项目(07ZCKFNC00200)

Preparation of Lactoferrin – Chromium Complex

WU Yang,HU Zhi-he*   

  1. Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin
    300134, China
  • Received:2009-07-08 Revised:2009-11-19 Online:2010-04-15 Published:2010-12-29
  • Contact: HU Zhi-he* E-mail:hzihe@tjcu.edu.cn

摘要:

以乳铁蛋白和三氯化铬为主要原料,制备乳铁蛋白铬。比较含铁乳铁蛋白、脱铁乳铁蛋白与Cr3+ 络合的条件,采用红外吸收光谱法进行结构表征,并用火焰原子吸收光谱法测定Fe3+、Cr3+ 含量。结果表明,含铁乳铁蛋白与Cr3+ 络合的适宜条件为温度范围50~60℃、pH6.5~7.5、络合时间2h 以上,铬饱和度90% 以上;脱铁乳铁蛋白与Cr3+ 络合的适宜条件为温度范围50~65℃、pH6.0~7.5、络合时间2h 以上,铬饱和度接近100%。可见,脱铁乳铁蛋白铬的络合范围比含铁乳铁蛋白铬的络合范围更广,铬饱和度更高。

关键词: 乳铁蛋白, 三氯化铬, 乳铁蛋白铬, 制备

Abstract:

Lactoferrin–chromium complexes were prepared by complexation of lactoferrin with chromium trichloride. The optimal complexation conditions between either ferric lactoferrin or apo-lactoferrin and Cr3+ were studied. The complexes formed were structurally characterized by infrared spectroscopy and analyzed for the contents of Fe3+ and Cr3+ by flame atomic absorption spectroscopy. Results showed that a maximum chromium saturation point of more than 90% was achieved through the complexation reaction between ferric lactoferrin and Cr3+ for more than 2 h at 50-60 ℃ and pH 6.5-7.5 and the complexation reaction between apo-lactoferrin and Cr3+ for more than 2 h at 50-65 ℃ and pH 6.0-7.5 gave a maximum chromium saturation point of close to 100%. Therefore, apo-lactoferrin is more suitable for preparation of lactoferrin-chromium complex.

Key words: lactoferrin, chromium trichloride, lactoferrin-chromium complex, preparation

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