食品科学 ›› 2010, Vol. 31 ›› Issue (6): 196-198.doi: 10.7506/spkx1002-6630-201006045

• 分析检测 • 上一篇    下一篇

槟榔嫩果中儿茶素的液相色谱质谱联用测定

王明月1 ,2,罗金辉2,赵振东3   

  1. 1.中国热带农业科学院热带生物技术研究所 2.中国热带农业科学院分析测试中心 3.海南大学分析测试中心
  • 收稿日期:2009-07-01 修回日期:2009-10-21 出版日期:2010-03-15 发布日期:2010-12-29
  • 通讯作者: 王明月 E-mail:guihongxing@263.net
  • 基金资助:

    2008 年中央级公益性科研院所基本科研业务费项目(ITBBKF083)

HPLC-MS Determination of Catechins in Tender Areca Nut

WANG Ming-yue1,2,LUO Jin-hui2,ZHAO Zhen-dong3   

  1. 1. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101,
    China ;2. Analysis and Testing Center, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China;
    3. Analysis and Testing Center, Hainan University, Haikou 570228, China
  • Received:2009-07-01 Revised:2009-10-21 Online:2010-03-15 Published:2010-12-29
  • Contact: WANG Ming-yue1 E-mail:guihongxing@263.net

摘要:

采用甲醇超声提取槟榔嫩果中的儿茶素,先用石油醚除去杂质,再用乙酸乙酯萃取,提取物经抽真空浓缩,高效液相色谱- 质谱联用法测定。结果表明:槟榔嫩果中儿茶素类组分为(+)- 儿茶素和表没食子儿茶素没食子酸酯。

关键词: 高效液相色谱-质谱法, 槟榔嫩果, 儿茶素

Abstract:

An Agilent HPLC-MS system was used to determine catechins in tender areca nut. Sample preparation was achieved through methanol extraction with the assistance of ultrasonic, petroleum ether extraction for the removal of impurities, ethyl acetate extraction and vacuum concentration. Results showed that catechins in tender areca nut mainly comprised (+) - catechin and epigallocatechin gallate.

Key words: high-performance liquid chromatography-mass spectrometry (HPLC-MS), tender areca nut, catechins

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