Abstract:

AB-8, DM301 and NKA-9 resins were screened from 7 types of macroporous resin in terms of adsorption and desorption capacities to total flavonoids from peanut hull for further studies of static adsorption and desorption kinetics. NKA-9 resin was finally chosen for the purification due to better adsorption and desorption performance, whose equilibrium time of adsorption and desorption were 5 and 2 h, respectively. Single factor experiments showed that the optimum purification procedure of total flavonoids from peanut hull was based on adsorption at 35 ℃of sample solution with an initial concentration of 0.112 ± 0.02 mg/ml at pH 7.5, followed by desorption using 90% aqueous ethanol (V/V) at pH 8.5 with an amount of 15 ml/g (wet resin).

Key words: macroporous resin, peanut hull, total flavonoids, purification