Abstract:

This work studied static and dynamic adsorption and desorption of total flavonoids prepared from pagoda flowers by microwave-assisted extraction on macropurous resin AB-8. Results showed that AB-8 resin had good adsorption capacity to pagoda flower flavonoids; the optimum static adsorption and desoprtion conditions were as follows: adsorption on a shaking table for 80 min at 25 ℃with a ratio of resin to sample 1:20(g/ml), at pH 5－6, sample concentration 0.10－0.17 mg/ml and then desorption with 70% aqueous ethanol as eluent, and under such conditions, the recovery of flavonoids was 71.94%; a recovery of total flavonoids of 39.32% was obtained by the following optimum dynamic adsorption and desoption conditions of sample concentration 0.045－0.070 mg/ml, pH 5, ratio of column diameter to length 1:8, flow rate 0.2 ml/min and 70% aqueous ethanol as eluent.

Key words: macroporous resin, pagoda flowers, total flavonoid, adsorption