FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (20): 169-172.doi: 10.7506/spkx1002-6300-200920033

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Synthesis of Sudan I Analog and Preparation of its Artificial Antigen

MENG Wei,LIU Ren-rong*,QIU Xue-mei,TU Shun,XIE Shao-xia,WU Qian-hui   

  1. College of Life Science, Jiangxi Science and Technology Normal University, Nanchang 330013, China
  • Received:2008-11-28 Revised:2009-07-06 Online:2009-10-15 Published:2010-12-29
  • Contact: LIU Ren-rong*, E-mail:lilirenrong@hotmail.com

Abstract:

A Sudan I analog was synthesized by diazotization and its artificial antigen was prepared by conjugating a carrier protein to the Sudan I analog using an active ester method. Immunogenity of the artificial antigen was determined through a competitive enzyme-linked immunosorbent assay (ELISA). UV, FT-IR and NMR spectra exhibited a successful synthesis of Sudan I analog and an effective coupling to the carrier protein. Antibodies against Sudan I were generated in serum of immunized BALB/c mice with prepared artificial antigen. All of these investigations revealed a successful preparation of Sudan I artificial antigen.

Key words: Sudan I, artificial antigen, ELISA

CLC Number: