FOOD SCIENCE ›› 2017, Vol. 38 ›› Issue (13): 299-305.doi: 10.7506/spkx1002-6630-201713048

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Recent Advances in Application of Immunological Methods in Detection of Foodborne Vibiro parahaemolyticus

XIE Xueqin, LIU Zhou   

  1. 1. Xiamen Products Quality Supervision & Inspection Institute, Xiamen 361004, China;2. Depatment of Pharmacy, Xiamen Medical College, Xiamen 361008, China
  • Online:2017-07-15 Published:2017-07-11

Abstract: The bacterial pathogen Vibrio parahaemolyticus (VP) can pollute a variety of foods, especially seafoods. Foodborne VP has surged as the primary pathogen in food safety incidents in China. Compared to traditional cultural methods and modern molecular techhniques, specific antigen-antibody recognition-based immunological identification and subtyping strategies exhibit superiority in efficiency, specificity, on-field applicability and simplicity of pre-treatment. In this paper, the category and preparation methods of anti-VP antibodies at present are outlined. Furthermore, we review the current applications of immunological methods such as immunomagnetic separation (IMS), enzyme-linked immunosorbent assay (ELISA), immunochromatographic assay (ICA), immunosensor and immunomicroscopy in the detection of foodborne VP, which will provide useful references for rapid detection of VP.

Key words: foodborne Vibrio parahaemolyticus, immunomagnetic separation (IMS), enzyme-linked immunosorbent assay (ELISA), immunochromatographic assay (ICA), immunosensor, immunofluorescence microscopy (IFM)

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