FOOD SCIENCE

• Bioengineering • Previous Articles     Next Articles

Preparation and Application of Monoclonal Antibody against Furazolidone

CHEN Yinnan1, CHEN Hua1, SHI Xianai1,2,*, YE Xiaojun3, FAN Haiping3   

  1. 1. College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, China;
    2. Fujian Key Laboratory of Medical Instrument and Pharmaceutical Technology, Fuzhou 350002, China;
    3. Fresh Water Fisheries Research Institute of Fujian Province, Fuzhou 350002, China
  • Online:2016-02-15 Published:2016-02-26

RichHTML

3

PDF (PC)

262

Abstract:

Based on the hapten of furazolidone (FZD), a monoclonal (mAb)-based enzyme-linked immunosorbent assay
(ELISA) method for the detection of FZD was established after the preparation of mAb against FZD-bovine serum albumin
(BSA). The linear range for furazolidone detection was 10–500 ng/mL, and the IC50 was 0.06 μg/mL with a lowest detection
limit of 6.92 ng/mL. Meanwhile, the mAb showed almost no cross reactivity with other nitrofurans or their metabolites. This
method had good reproducibility, with an average error of 6.54%. In tested samples, the recovery for furazolidone addition
was 76.84%–88.31%.

Key words: furazolidone (FZD), monoclonal antibody (mAb), enzyme-linked immunosorbent assay (ELISA)

CLC Number: