Abstract:

Based on the haptens of the furazolidone metabolite 3-amino-2-oxazolidinone (AOZ) and its derivative CPAOZ,
mAb-based ELISA kits for the detection of AOZ were established after the preparation of monoclonal antibodies (mAbs)
against AOZ-BSA. The CPAOZ-based ELISA kit indicated excellent linearity over the range of 1.0–100.0 ng/mL, The IC50
was 14.6 ng/mL, with detection limit of 6.56 ng/mL, and the recovery for CPAOZ was 97.6%–101.3%. The AOZ-based
ELISA kit exhibited excellent linearty over the range of 0.5–12.5 ng/mL, The IC50 was 3.9 ng/mL, with the detection limit
of 0.45 ng/mL, and the recovery for AOZ was 94.1%–97.4%. Neither of the ELISA kits showed cross reactivity with other
nitrofurans or their metabolites. Obviously, the AOZ-based ELISA kit has more promising prospects due to its advantages of
economy, convenience, rapidity and sensitivity.

Key words: 3-amino-2-oxazolidinone (AOZ), hapten, monoclonal antibody (mAb), enzyme-linked immunosorbent assay (ELISA)