Abstract:

This work aimed to develop an effective novel method to synthesize artificial antigens of forchlorfenuron
(CPPU). Towards this goal, Friedel-Crafts reaction was used to modify the structure of forchlorfenuron with the catalysis
of anhydrous aluminum chloride and the hapten was coupled with bovine serum albumin (BSA) and ovalbumin (OVA)
by carbondiimine (EDC) method, respectively, to obtain forchlorfenuron immunogen and coating antigen. The molecular
structure of the forchlorfenuron hapten was identified by elemental analysis, mass spectroscopy (MS) and nuclear magnetic
resonance spectroscopy (1H-NMR). The artificial antigens were identified by UV spectroscopy and high performance
matrix assisted laser desorption ionization time of flight mass spectroscopy (MALDI-TOF-MS). The experimental results
showed that the artificial antigen of forchlorfenuron has been synthesized successfully and it can lay the foundation for the
preparation of antibody and the development of immunoassays.

Key words: forchlorfenuron, hapten, artificial antigen, synthesis, identification