Abstract:

An electrochemical sensing method for the detection of clenbuterol (CLB) was developed based on miniscreen
printed electrodes and 96-well microtiter plate. Clenbuterol residues were indirectly detected by combined use of
indirect competitive immunoreaction and chronoamperometry. The immune reaction and enzymatic reaction conditions
were investigated. Under the optimized conditions, the results showed that the electrochemical immunosensor produced a
linear range of 0.025–2.0 μg/L with a correlation coefficient of 0.999 2. The analytical results of CLB residues in pork and
swine urine samples were consistent with those obtained with an enzyme-linked immunosorbent assay (ELISA) plate. The
detection limits for pork and swine urine samples were 0.18 μg/L and 0.05 μg/L, respectively, showing a decrease of one
order of magnitude in comparison with those of ELISA.

Key words: mini-screen printed electrode, clenbuterol, chronoamperometry, electrochemical immunosensor