Abstract: The objectives of this study were to develop a preparation method for amino-polystyrene microspheres and to evaluate its application in purification of antibodies against clenbuterol. Polystyrene was prepared by dispersion polymerization, and converted into polystyrene microspheres by nitration reaction, which were reduced into amino-microspheres. Scanning electron microscopy and infrared spectrometry was used to characterize the shape and structure of microspheres. Amino-polystyrene microspheres were bound with clenbuterol molecules by diazotization reaction. Amino-polystyrene microspheres with clenbuterol were applied in the immunoaffinity adsorption and separation of anti-clenbuterol antibodies. Finally, purified antibodies were detected by UV and electrophoresis assay. We proved that it is feasible to use amino-polystyrene microspheres with clenbuterol prepared in this study to purify anti-clenbuterol antibodies.

Key words: polystyrene, clenbuterol, antibody purification, immunoaffinity