FOOD SCIENCE ›› 2017, Vol. 38 ›› Issue (6): 315-320.doi: 10.7506/spkx1002-6630-201706049

• Safety Detection • Previous Articles    

Uncertainty Evaluation for the Determination of β-Agonists in Pork by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

VNING Xiao, JIN Shaoming, LIANG Ruiqiang, CAO Jin, ZHANG Qingsheng   

  1. National Institutes for Food and Drug Control, Beijing 100050, China
  • Received:2017-03-30 Revised:2017-03-30 Online:2017-03-25 Published:2017-03-28

Abstract: This study aimed to evaluate the uncertainty of measurement in the determination of salbutamol, clenbuterol hydrochloride and ractompaine in pork by ultra performance liquid chromatography-tandem mass spectrometry (UPLCMS/ MS). According to JJF 1135-2005 Evaluation of Uncertainty in Chemical Analysis Measurement and JJF 1059.1-2012 Evaluation and Expression of Uncertainty in Measurement, a mathematical model was established for uncertainty evaluation. The sources of uncertainty that may be introduced were analyzed and each component of uncertainty was quantified for the calculation of the combined uncertainty. The results showed that an expanded uncertainty of 0.25 μg/kg (k = 2) was obtained for the determination of the presence of 1.99 μg/kg salbutamol in pork, the expanded uncertainty was 0.24 μg/kg (k = 2) for the determination of pork samples containing 2.04 and 1.97 μg/kg salbutamol. The preparation of standard solution, curve fitting and measurement repeatability were found to be the main sources of uncertainty.

Key words: ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), salbutamol, clenbuterol hydrochloride, ractompaine, uncertainty, pork

CLC Number: