Development of Immunoassay for Detection of Chloramphenicol

doi:10.7506/spkx1002-6300-200920094

Abstract

Abstract:

Immunogen and coating antigens of chloramphenicol were successfully synthesized through coupling with BSA and OVA using the method of EDC and mixed anhydride. The conjugation was evaluated using UV spectroscopy and SDS -PAGE, and the conjugation rates were estimated to be 1:17 and 1:16, respectively. Two New Zealand rabbits were immuned by CAP-HS-BSA to generate polyclonal antibody. The titers of both antibodies were 218700 and 72900, respectively, which reached the standard of enzyme linked immunosorbent assay (ELISA). An indirect competitive ELISA was developed to determine chloramphenicol using these antibodies. The IC50 for chloramphenicol was 0.86 μg/L using ELISA determination. The specificity of chloramphenicol sodium succinate, streptomycin, penicillin and gentamicin was evaluated using cross reactivity, which revealed that the developed antibody had high specificity to chloramphenicol sodium succinate, while low specificity to other three drugs.

Key words: chloramphenicol, IC-ELISA, residue

CLC Number:

S852.43

HU Yong-ming，LIANG Sha-li，XU Li-guang，CHEN Wei，WANG Li-bing，XU Chuan-lai*. Development of Immunoassay for Detection of Chloramphenicol[J]. FOOD SCIENCE, 2009, 30(20 ): 416-420.

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Development of Immunoassay for Detection of Chloramphenicol

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