Prediction of Secondary Structure and B Cell Epitopes of Peanut Allergen Ara h 2.02

doi:10.7506/spkx1002-6300-200921001

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (21 ): 13-15.doi: 10.7506/spkx1002-6300-200921001

Prediction of Secondary Structure and B Cell Epitopes of Peanut Allergen Ara h 2.02

HU Chun-qiu1,2，GAO Jin-yan1,3，LUO Chun-ping1,2，CHEN Hong-bing1,2,*

1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China；
2. Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China；
3. School of Life Sciences and Food Engineering, Nanchang University, Nanchang 330047, China

Received:2009-07-04 Online:2009-11-01 Published:2010-12-29
Contact: CHEN Hong-bing1,2,* E-mail:chbgjy@hotmail.com

Abstract

Abstract:

In order to predict the secondary structure and B cell epitopes of peanut allergen Ara h 2.02, the gene of Ara h 2.02 was searched in Genbank and its amino sequence was deduced. Meanwhile, its secondary structure was predicted with DNAStar and SOMPA, and antigenicity. hydrophilicity, surface probability and flexibility was predicted using the methods of Jameson- Wolf, Kyte-Doolittle, Emini and Karplus-eSchulz, respectively. B-cell epitopes of Ara h 2.02 were further predicted based on the comprehensive prediction analysis. Results indicated that B-cell epitopes had the high probability in the regions of 28－31, 56－73, 76－90, 92, 148, 156－158, and 168－169. These investigations will be beneficial to epitope localization of Ara h 2.02 and monoclonal antibody preparation in the future.

Key words: Ara h 2.02, peanut allergy, secondary structure, B-cell epitopes

CLC Number:

Q518.1

HU Chun-qiu1,2，GAO Jin-yan1,3，LUO Chun-ping1,2，CHEN Hong-bing1,2,*. Prediction of Secondary Structure and B Cell Epitopes of Peanut Allergen Ara h 2.02[J]. FOOD SCIENCE, 2009, 30(21 ): 13-15.

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Prediction of Secondary Structure and B Cell Epitopes of Peanut Allergen Ara h 2.02

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