Mechanism and Kinetic Model for Limited Trypsin Digestion of Protein in Rice Dregs

doi:10.7506/spkx1002-6300-200921039

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (21 ): 166-171.doi: 10.7506/spkx1002-6300-200921039

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Mechanism and Kinetic Model for Limited Trypsin Digestion of Protein in Rice Dregs

LI Xiang1,2，PENG Di-wei1,2，XIONG Hua1,2,*，ZHAO Qiang1,2，LI Wei1,2

1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China；
2. College of Life Science and Food Engineering, Nanchang University, Nanchang 330047, China

Received:2009-06-30 Online:2009-11-01 Published:2010-12-29
Contact: XIONG Hua1,2,*， E-mail:huaxiong100@yahoo.com.cn

Abstract

Abstract:

Mechanisms and kinetic models of enzymatic hydrolysis of protein in rice dregs by trypsin at pH 7.6 and 53 ℃ were investigated using the combined method of experimental analysis and mathematic deduction. Results indicated that the overall rate of hydrolysis decreased exponentially during the hydrolysis process due to dual functions of the substrate for accelerating and inhibiting enzymatic activity. Based on experimental data, a kinetic model equation was deduced to demonstrate trypsin hydrolysis of protein in rice dregs and inactivation constant of trypsin. Therefore, hydrolysis degree can be effectively controlled through the adjustment of trypsin/substrate ratio, and reaction time, which can provide guidance to peptide industrialization from rice dregs by means of enzymatic technology.

Key words: rice dregs, trypsin, limited-hydrolysis, kinetic model

CLC Number:

TS201.25

LI Xiang1,2，PENG Di-wei1,2，XIONG Hua1,2,*，ZHAO Qiang1,2，LI Wei1,2. Mechanism and Kinetic Model for Limited Trypsin Digestion of Protein in Rice Dregs[J]. FOOD SCIENCE, 2009, 30(21 ): 166-171.

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Mechanism and Kinetic Model for Limited Trypsin Digestion of Protein in Rice Dregs

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